Chapter 37 : Mycobacterial Biofilms

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In a review published in 1896 on early bacteriological studies of the tubercle bacilli, , A. Coppen Jones ( ) wrote:

Citation: Richards J, Ojha A. 2014. Mycobacterial Biofilms, p 773-784. In Hatfull G, Jacobs W (ed), Molecular Genetics of Mycobacteria, Second Edition. ASM Press, Washington, DC. doi: 10.1128/microbiolspec.MGM2-0004-2013
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Figure 1

A schematic representation of distinct developmental stages of microbial biofilms. Transition from one stage has specific genetic requirements. This scheme was originally published by the authors in ( ) and is reproduced here in accordance with the publisher’s policy.

Citation: Richards J, Ojha A. 2014. Mycobacterial Biofilms, p 773-784. In Hatfull G, Jacobs W (ed), Molecular Genetics of Mycobacteria, Second Edition. ASM Press, Washington, DC. doi: 10.1128/microbiolspec.MGM2-0004-2013
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Image of Figure 2
Figure 2

Various models of mycobacterial biofilms grown in our laboratory. Pellicles biofilms of on air-liquid interface in a petri dish. Scanning electron micrograph of flow-cell biofilms of on silicon surface, developed against the shear fluid flow of 1 ml/minute. Pellicle biofilms of in syringes (marked by arrow). This technique is amenable to screening mutants that remain exclusively in planktonic suspension beneath biofilms. Pellicle biofilms of on liquid-air interface grown in a 12-well plate. Scanning electron micrograph of biofilms grown on the surface of a polycarbonate membrane. Images in panels and were generated with help from Curtis Larimer and Ian Nettleship from the Swanson School of Engineering, University of Pittsburgh.

Citation: Richards J, Ojha A. 2014. Mycobacterial Biofilms, p 773-784. In Hatfull G, Jacobs W (ed), Molecular Genetics of Mycobacteria, Second Edition. ASM Press, Washington, DC. doi: 10.1128/microbiolspec.MGM2-0004-2013
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Figure 3

A representative structure of methoxy-free mycolic acids, predominantly accumulated in the bioflms of .

Citation: Richards J, Ojha A. 2014. Mycobacterial Biofilms, p 773-784. In Hatfull G, Jacobs W (ed), Molecular Genetics of Mycobacteria, Second Edition. ASM Press, Washington, DC. doi: 10.1128/microbiolspec.MGM2-0004-2013
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Figure 4

Biofilms of and harbor higher numbers of rifampin-tolerant persisters than their planktonic counterparts. The frequency of such persisters is diminished in the impaired biofilms of Δ. Data in panel were originally published in ( ) and reproduced here in accordance with the publisher’s policy.

Citation: Richards J, Ojha A. 2014. Mycobacterial Biofilms, p 773-784. In Hatfull G, Jacobs W (ed), Molecular Genetics of Mycobacteria, Second Edition. ASM Press, Washington, DC. doi: 10.1128/microbiolspec.MGM2-0004-2013
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