Chapter 15 : Molecular Detection of Colonization and Infection

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Prevention and control of infection with , including methicillin-resistant (MRSA), have been a major health care focus since at least the middle of the 20th century (1). By 2005, mortality from MRSA in the United States exceeded that due to infections from salmonella, tuberculosis, influenza, and HIV combined (2, 3). Since that time, global efforts to reduce MRSA infection rates appear to have had some impact in both Europe and the United States (4–6). However, the improvement has not been uniform, with selected academic medical centers not realizing a reduction in disease (7), and at least one large state (Illinois) reporting an increase in overall MRSA infection reflected in their most recent data, with 11.7 MRSA infections/1,000 hospital discharges in 2009, 11.6 in 2010, 10.7 in 2011, and then up to 14.2 in 2012 (8).

Citation: Mangold K, Peterson L. 2016. Molecular Detection of Colonization and Infection, p 169-184. In Persing D, Tenover F, Hayden R, Ieven M, Miller M, Nolte F, Tang Y, van Belkum A (ed), Molecular Microbiology. ASM Press, Washington, DC. doi: 10.1128/9781555819071.ch15
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Commercial assays for MRSA/MSSA molecular testing

Citation: Mangold K, Peterson L. 2016. Molecular Detection of Colonization and Infection, p 169-184. In Persing D, Tenover F, Hayden R, Ieven M, Miller M, Nolte F, Tang Y, van Belkum A (ed), Molecular Microbiology. ASM Press, Washington, DC. doi: 10.1128/9781555819071.ch15
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Analytic performance of commercial assays for MRSA/MSSA molecular testing

Citation: Mangold K, Peterson L. 2016. Molecular Detection of Colonization and Infection, p 169-184. In Persing D, Tenover F, Hayden R, Ieven M, Miller M, Nolte F, Tang Y, van Belkum A (ed), Molecular Microbiology. ASM Press, Washington, DC. doi: 10.1128/9781555819071.ch15
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Laboratory workflow for commercial assays for MRSA/MSSA molecular testing

Citation: Mangold K, Peterson L. 2016. Molecular Detection of Colonization and Infection, p 169-184. In Persing D, Tenover F, Hayden R, Ieven M, Miller M, Nolte F, Tang Y, van Belkum A (ed), Molecular Microbiology. ASM Press, Washington, DC. doi: 10.1128/9781555819071.ch15
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Comparison of health care-associated infection outcome of universal decolonization ( ) with concurrent data from NorthShore University HealthSystem (authors' unpublished data)

Citation: Mangold K, Peterson L. 2016. Molecular Detection of Colonization and Infection, p 169-184. In Persing D, Tenover F, Hayden R, Ieven M, Miller M, Nolte F, Tang Y, van Belkum A (ed), Molecular Microbiology. ASM Press, Washington, DC. doi: 10.1128/9781555819071.ch15

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