Chapter 33 : Molecular Detection of Human Papillomaviruses

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The importance of human papillomaviruses (HPVs) in biology and medicine was highlighted by the award of the 2008 Nobel Prize in Medicine to Harald zur Hausen in recognition of his discovery of the oncogenic role of HPV in cervical cancer (1). Numerous international epidemiologic studies confirmed this association, which ultimately led to the successful development of vaccines that prevent HPV infection and tests that provide another tool for cervical cancer control.

Citation: Quigley D, Unger E. 2016. Molecular Detection of Human Papillomaviruses, p 465-475. In Persing D, Tenover F, Hayden R, Ieven M, Miller M, Nolte F, Tang Y, van Belkum A (ed), Molecular Microbiology. ASM Press, Washington, DC. doi: 10.1128/9781555819071.ch33
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Image of FIGURE 1

Schematic diagram of genomic organization of the open reading frame of human papillomavirus 16. P97 refers to dominant promoter. All papillomaviruses are similar in their genetic organization and appearance by electron microscopy. URR, upstream regulatory region.

Citation: Quigley D, Unger E. 2016. Molecular Detection of Human Papillomaviruses, p 465-475. In Persing D, Tenover F, Hayden R, Ieven M, Miller M, Nolte F, Tang Y, van Belkum A (ed), Molecular Microbiology. ASM Press, Washington, DC. doi: 10.1128/9781555819071.ch33
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Image of FIGURE 2

Phylogenetic tree based on the L1 open reading frame sequences of 170 HPV types, single animal papillomaviruses, and newly identified HPVs using metagenomic sequencing. The maximum likelihood method was used for the analysis and the tree was constructed using the MEGA5.1 program. The numbers at the ends of each of the branches identify an HPV type; m-numbers refer to metagenomics. All other abbreviations refer to animal PV types. The outermost semicircular symbols identify PV genera (e.g., the genus ) The number at the inner semicircular symbol refers to PV species. To give an example taken from the upper part of the figure, the HPV types 33, 58, 67, 52, 31, 16, and 35 together form the HPV species 9 in the genus . Reprinted from de Villiers ( ) with permission of the publisher.

Citation: Quigley D, Unger E. 2016. Molecular Detection of Human Papillomaviruses, p 465-475. In Persing D, Tenover F, Hayden R, Ieven M, Miller M, Nolte F, Tang Y, van Belkum A (ed), Molecular Microbiology. ASM Press, Washington, DC. doi: 10.1128/9781555819071.ch33
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Generic image for table

Summary of clinical tests for high-risk human papillomavirus detection discussed in this chapter

Citation: Quigley D, Unger E. 2016. Molecular Detection of Human Papillomaviruses, p 465-475. In Persing D, Tenover F, Hayden R, Ieven M, Miller M, Nolte F, Tang Y, van Belkum A (ed), Molecular Microbiology. ASM Press, Washington, DC. doi: 10.1128/9781555819071.ch33

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