Chapter 34 : Molecular Diagnostics for Viral Infections in Transplant Recipients

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Viral infections are some of the most common complications in transplant recipients as a result of these patients' immunocompromised status. The ability to accurately detect and identify viral pathogens in transplant patients is important to tailor therapy and improve outcomes. In this chapter, we review the role of molecular tests in the diagnosis and management of human adenovirus (HAdV), BK virus (BKV), cytomegalovirus (CMV), Epstein-Barr virus (EBV), human herpesvirus 6 (HHV-6), herpes simplex virus (HSV), parvovirus B19, and varicella-zoster virus (VZV) in hematopoietic stem cell transplant (HSCT) and solid-organ transplant (SOT) recipients.

Citation: Binnicker M, Razonable R. 2016. Molecular Diagnostics for Viral Infections in Transplant Recipients, p 476-486. In Persing D, Tenover F, Hayden R, Ieven M, Miller M, Nolte F, Tang Y, van Belkum A (ed), Molecular Microbiology. ASM Press, Washington, DC. doi: 10.1128/9781555819071.ch34
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1. Heim A . 2011. Advances in the management of disseminated adenovirus disease in stem cell transplant recipients: impact of adenovirus load (DNAemia) testing. Expert Rev Anti Infect Ther 9 : 943 945[CrossRef].[PubMed]
2. Seidemann K, Heim A, Pfister ED, Köditz H, Beilken A, Sander A, Melter M, Sykora KW, Sasse M, Wessel A . 2004. Monitoring of adenovirus infection in pediatric transplant recipients by quantitative PCR: report of six cases and review of the literature. Am J Transplant 4 : 2102 2108[CrossRef].[PubMed]
3. Ison MG . 2006. Adenovirus infections in transplant recipients. Clin Infect Dis 43 : 331 339[CrossRef].[PubMed]
4. Suparno C, Milligan DW, Moss PA, Mautner V . 2004. Adenovirus infections in stem cell transplant recipients: recent developments in understanding of pathogenesis, diagnosis and management. Leuk Lymphoma 45 : 873 885[CrossRef].[PubMed]
5. Flomenberg P, Babbitt J, Drobyski WR, Ash RC, Carrigan DR, Sedmak GV, McAuliffe T, Camitta B, Horowitz MM, Bunin N, Casper JT . 1994. Increasing incidence of adenovirus disease in bone marrow transplant recipients. J Infect Dis 169 : 775 781[CrossRef].[PubMed]
6. Buckwalter SP, Teo R, Espy MJ, Sloan LM, Smith TF, Pritt BS . 2012. Real-time qualitative PCR for 57 human adenovirus types from multiple specimen sources. J Clin Microbiol 50 : 766 771[CrossRef].[PubMed]
7. Heim A, Ebnet C, Harste G, Pring-Akerblom P . 2003. Rapid and quantitative detection of human adenovirus DNA by real-time PCR. J Med Virol 70 : 228 239[CrossRef].[PubMed]
8. Manji R, Zheng X, Patel A, Kowerska M, Vossinas M, Drain A, Todd KM, Lenny N, DeVincenzo JP, Ginocchio CC . 2014. Multi-center evaluation of the adenovirus R-gene US assay for the detection of adenovirus in respiratory samples. J Clin Virol 60 : 90 95[CrossRef].[PubMed]
9. Claas EC, Schilham MW, de Brouwer CS, Hubacek P, Echavarria M, Lankester AC, van Tol MJ, Kroes AC . 2005. Internally controlled real-time PCR monitoring of adenovirus DNA load in serum or plasma of transplant recipients. J Clin Microbiol 43 : 1738 1744[CrossRef].[PubMed]
10. Ebner K, Suda M, Watzinger F, Lion T . 2005. Molecular detection and quantitative analysis of the entire spectrum of human adenoviruses by a two-reaction real-time PCR assay. J Clin Microbiol 43 : 3049 3053[CrossRef].[PubMed]
11. Leruez-Ville M, Minard V, Lacaille F, Buzyn A, Abachin E, Blanche S, Freymuth F, Rouzioux C . 2004. Real-time blood plasma polymerase chain reaction for management of disseminated adenovirus infection. Clin Infect Dis 38 : 45 52[CrossRef].[PubMed]
12. Rux JJ, Kuser PR, Burnett RM . 2003. Structural and phylogenetic analysis of adenovirus hexons by use of high-resolution x-ray crystallographic, molecular modeling, and sequence-based methods. J Virol 77 : 9553 9566[CrossRef].[PubMed]
13. Scott-Taylor TH, Hammond GW . 1992. Conserved sequences of the adenovirus genome for detection of all human adenovirus types by hybridization. J Clin Microbiol 30 : 1703 1710.[PubMed]
14. Schilham MW, Claas EC, van Zaane W, Heemskerk B, Vossen JM, Lankester AC, Toes RE, Echavarria M, Kroes AC, van Tol MJ . 2002. High levels of adenovirus DNA in serum correlate with fatal outcome of adenovirus infection in children after allogeneic stem-cell transplantation. Clin Infect Dis 35 : 526 532[CrossRef].[PubMed]
15. Goudsmit J, Wertheim-van Dillen P, van Strien A, van der Noordaa J . 1982. The role of BK virus in acute respiratory tract disease and the presence of BKV DNA in tonsils. J Med Virol 10 : 91 99[CrossRef].[PubMed]
16. Reploeg MD, Storch GA, Clifford DB . 2001. Bk virus: a clinical review. Clin Infect Dis 33 : 191 202[CrossRef].[PubMed]
17. Hirsch HH, Steiger J . 2003. Polyomavirus BK. Lancet Infect Dis 3 : 611 623[CrossRef].[PubMed]
18. Sencer SF, Haake RJ, Weisdorf DJ . 1993. Hemorrhagic cystitis after bone marrow transplantation. Risk factors and complications. Transplantation 56 : 875 879[CrossRef].[PubMed]
19. Yang CC, Hurd DD, Case LD, Assimos DG . 1994. Hemorrhagic cystitis in bone marrow transplantation. Urology 44 : 322 328[CrossRef].[PubMed]
20. Bogdanovic G, Priftakis P, Taemmeraes B, Gustafsson A, Flaegstad T, Winiarski J, Dalianis T . 1998. Primary BK virus (BKV) infection due to possible BKV transmission during bone marrow transplantation is not the major cause of hemorrhagic cystitis in transplanted children. Pediatr Transplant 2 : 288 293.[PubMed]
21. Chan PK, Ip KW, Shiu SY, Chiu EK, Wong MP, Yuen KY . 1994. Association between polyomaviruria and microscopic haematuria in bone marrow transplant recipients. J Infect 29 : 139 146[CrossRef].[PubMed]
22. Cottler-Fox M, Lynch M, Deeg HJ, Koss LG . 1989. Human polyomavirus: lack of relationship of viruria to prolonged or severe hemorrhagic cystitis after bone marrow transplant. Bone Marrow Transplant 4 : 279 282.[PubMed]
23. Leung AY, Suen CK, Lie AK, Liang RH, Yuen KY, Kwong YL . 2001. Quantification of polyoma BK viruria in hemorrhagic cystitis complicating bone marrow transplantation. Blood 98 : 1971 1978[CrossRef].[PubMed]
24. Priftakis P, Bogdanovic G, Kokhaei P, Mellstedt H, Dalianis T . 2003. BK virus (BKV) quantification in urine samples of bone marrow transplanted patients is helpful for diagnosis of hemorrhagic cystitis, although wide individual variations exist. J Clin Virol 26 : 71 77[CrossRef].[PubMed]
25. Gardner SD, Field AM, Coleman DV, Hulme B . 1971. New human papovavirus (B.K.) isolated from urine after renal transplantation. Lancet 1 : 1253 1257[CrossRef].[PubMed]
26. Purighalla R, Shapiro R, McCauley J, Randhawa P . 1995. BK virus infection in a kidney allograft diagnosed by needle biopsy. Am J Kidney Dis 26 : 671 673[CrossRef].[PubMed]
27. Hirsch HH, Brennan DC, Drachenberg CB, Ginevri F, Gordon J, Limaye AP, Mihatsch MJ, Nickeleit V, Ramos E, Randhawa P, Shapiro R, Steiger J, Suthanthiran M, Trofe J . 2005. Polyomavirus-associated nephropathy in renal transplantation: interdisciplinary analyses and recommendations. Transplantation 79 : 1277 1286[CrossRef].[PubMed]
28. Ramos E, Drachenberg CB, Papadimitriou JC, Hamze O, Fink JC, Klassen DK, Drachenberg RC, Wiland A, Wali R, Cangro CB, Schweitzer E, Bartlett ST, Weir MR . 2002. Clinical course of polyoma virus nephropathy in 67 renal transplant patients. J Am Soc Nephrol 13 : 2145 2151[CrossRef].[PubMed]
29. Ramos E, Drachenberg CB, Portocarrero M, Wali R, Klassen DK, Fink JC, Farney A, Hirsch H, Papadimitriou JC, Cangro CB, Weir MR, Bartlett ST . 2002. BK virus nephropathy diagnosis and treatment: experience at the University of Maryland Renal Transplant Program. Clin Transpl 2002 : 143 153.[PubMed]
30. Drachenberg RC, Drachenberg CB, Papadimitriou JC, Ramos E, Fink JC, Wali R, Weir MR, Cangro CB, Klassen DK, Khaled A, Cunningham R, Bartlett ST . 2001. Morphological spectrum of polyoma virus disease in renal allografts: diagnostic accuracy of urine cytology. Am J Transplant 1 : 373 381[CrossRef].[PubMed]
31. Hirsch HH, Knowles W, Dickenmann M, Passweg J, Klimkait T, Mihatsch MJ, Steiger J . 2002. Prospective study of polyomavirus type BK replication and nephropathy in renal-transplant recipients. N Engl J Med 347 : 488 496[CrossRef].[PubMed]
32. Bressollette-Bodin C, Coste-Burel M, Hourmant M, Sebille V, Andre-Garnier E, Imbert-Marcille BM . 2005. A prospective longitudinal study of BK virus infection in 104 renal transplant recipients. Am J Transplant 5 : 1926 1933[CrossRef].[PubMed]
33. Ding R, Medeiros M, Dadhania D, Muthukumar T, Kracker D, Kong JM, Epstein SR, Sharma VK, Seshan SV, Li B, Suthanthiran M . 2002. Noninvasive diagnosis of BK virus nephritis by measurement of messenger RNA for BK virus VP1 in urine. Transplantation 74 : 987 994[CrossRef].[PubMed]
34. Randhawa P, Ho A, Shapiro R, Vats A, Swalsky P, Finkelstein S, Uhrmacher J, Weck K . 2004. Correlates of quantitative measurement of BK polyomavirus (BKV) DNA with clinical course of BKV infection in renal transplant patients. J Clin Microbiol 42 : 1176 1180[CrossRef].[PubMed]
35. Cirocco R, Markou M, Rosen A, Goldsmith L, Cianco G, Roth D, Kupin W, Burke G, Esquenazi V, Tzakis A, Miller J . 2001. Polyomavirus PCR monitoring in renal transplant recipients: detection in blood is associated with higher creatinine values. Transplant Proc 33 : 1805 1807[CrossRef].[PubMed]
36. Limaye AP, Jerome KR, Kuhr CS, Ferrenberg J, Huang ML, Davis CL, Corey L, Marsh CL . 2001. Quantitation of BK virus load in serum for the diagnosis of BK virus-associated nephropathy in renal transplant recipients. J Infect Dis 183 : 1669 1672[CrossRef].[PubMed]
37. Nickeleit V, Klimkait T, Binet IF, Dalquen P, Del Zenero V, Thiel G, Mihatsch MJ, Hirsch HH . 2000. Testing for polyomavirus type BK DNA in plasma to identify renal-allograft recipients with viral nephropathy. N Engl J Med 342 : 1309 1315[CrossRef].[PubMed]
38. Tong CY, Hilton R, MacMahon EM, Brown L, Pantelidis P, Chrystie IL, Kidd IM, Tungekar MF, Pattison JM . 2004. Monitoring the progress of BK virus associated nephropathy in renal transplant recipients. Nephrol Dial Transplant 19 : 2598 2605[CrossRef].[PubMed]
39. Bechert CJ, Schnadig VJ, Payne DA, Dong J . 2010. Monitoring of BK viral load in renal allograft recipients by real-time PCR assays. Am J Clin Pathol 133 : 242 250[CrossRef].[PubMed]
40. Whiley DM, Mackay IM, Sloots TP . 2001. Detection and differentiation of human polyomaviruses JC and BK by LightCycler PCR. J Clin Microbiol 39 : 4357 4361[CrossRef].[PubMed]
41. Kohn D, Procop G, Yen-Lieberman B . 2010. A Preliminary Evaluation of Two Commercially Developed Quantitative BK Virus Assays. 26th Clinical Virology Symposium, Daytona Beach, FL.
42. Brennan DC, Agha I, Bohl DL, Schnitzler MA, Hardinger KL, Lockwood M, Torrence S, Schuessler R, Roby T, Gaudreault-Keener M, Storch GA . 2005. Incidence of BK with tacrolimus versus cyclosporine and impact of preemptive immunosuppression reduction. Am J Transplant 5 : 582 594[CrossRef].[PubMed]
43. Hirsch HH, Mohaupt M, Klimkait T . 2001. Prospective monitoring of BK virus load after discontinuing sirolimus treatment in a renal transplant patient with BK virus nephropathy. J Infect Dis Dec. 184( 11): 1494 5.
44. Vats A, Shapiro R, Singh Randhawa P, Scantlebury V, Tuzuner A, Saxena M, Moritz ML, Beattie TJ, Gonwa T, Green MD, Ellis D . 2003. Quantitative viral load monitoring and cidofovir therapy for the management of BK virus-associated nephropathy in children and adults. Transplantation 75 : 105 112[CrossRef].[PubMed]
45. Chen Y, Sharp PM, Fowkes M, Kocher O, Joseph JT, Koralnik IJ . 2004. Analysis of 15 novel full-length BK virus sequences from three individuals: evidence of a high intra-strain genetic diversity. J Gen Virol 85 : 2651 2663[CrossRef].[PubMed]
46. Nishimoto Y, Takasaka T, Hasegawa M, Zheng HY, Chen Q, Sugimoto C, Kitamura T, Yogo Y . 2006. Evolution of BK virus based on complete genome data. J Mol Evol 63 : 341 352[CrossRef].[PubMed]
47. Sharma PM, Gupta G, Vats A, Shapiro R, Randhawa P . 2006. Phylogenetic analysis of polyomavirus BK sequences. J Virol 80 : 8869 8879[CrossRef].[PubMed]
48. Hoffman NG, Cook L, Atienza EE, Limaye AP, Jerome KR . 2008. Marked variability of BK virus load measurement using quantitative real-time PCR among commonly used assays. J Clin Microbiol 46 : 2671 2680[CrossRef].[PubMed]
49. Arthurs SK, Eid AJ, Pedersen RA, Dierkhising RA, Kremers WK, Patel R, Razonable RR . 2007. Delayed-onset primary cytomegalovirus disease after liver transplantation. Liver Transpl 13 : 1703 1709[CrossRef].[PubMed]
50. Arthurs SK, Eid AJ, Pedersen RA, Kremers WK, Cosio FG, Patel R, Razonable RR . 2008. Delayed-onset primary cytomegalovirus disease and the risk of allograft failure and mortality after kidney transplantation. Clin Infect Dis 46 : 840 846[CrossRef].[PubMed]
51. García-Martínez J, Folgueira L, Delgado R, Hernando S, Prieto C, Aguado JM, Otero JR . 2008. Viral monitoring and successful treatment of a ganciclovir-resistant cytomegalovirus infection in a heart transplant recipient. Transpl Infect Dis 10 : 123 128[CrossRef].[PubMed]
52. Kleiboeker S, Nutt J, Schindel B, Dannehl J, Hester J . 2014. Cytomegalovirus antiviral resistance: characterization of results from clinical specimens. Transpl Infect Dis 16 : 561 567[CrossRef].[PubMed]
53. Rodriguez J, Casper K, Smallwood G, Stieber A, Fasola C, Lehneman J, Heffron T . 2007. Resistance to combined ganciclovir and foscarnet therapy in a liver transplant recipient with possible dual-strain cytomegalovirus coinfection. Liver Transpl 13 : 1396 1400[CrossRef].[PubMed]
54. Binnicker MJ, Espy ME . 2013. Comparison of six real-time PCR assays for qualitative detection of cytomegalovirus in clinical specimens. J Clin Microbiol 51 : 3749 3752[CrossRef].[PubMed]
55. Garrigue I, Doussau A, Asselineau J, Bricout H, Couzi L, Rio C, Merville P, Fleury H, Lafon ME, Thiébaut R . 2008. Prediction of cytomegalovirus (CMV) plasma load from evaluation of CMV whole-blood load in samples from renal transplant recipients. J Clin Microbiol 46 : 493 498[CrossRef].[PubMed]
56. Gerna G, Baldanti F, Torsellini M, Minoli L, Viganò M, Oggionnis T, Rampino T, Castiglioni B, Goglio A, Colledan M, Mammana C, Nozza F, Daniele L , Bergamo Transplant Group . 2007. Evaluation of cytomegalovirus DNAaemia versus pp65-antigenaemia cutoff for guiding preemptive therapy in transplant recipients: a randomized study. Antivir Ther 12 : 63 72.[PubMed]
57. Gerna G, Lilleri D, Caldera D, Furione M, Zenone Bragotti L, Alessandrino EP . 2008. Validation of a DNAemia cutoff for preemptive therapy of cytomegalovirus infection in adult hematopoietic stem cell transplant recipients. Bone Marrow Transplant 41 : 873 879[CrossRef].[PubMed]
58. Humar A, Gregson D, Caliendo AM, McGeer A, Malkan G, Krajden M, Corey P, Greig P, Walmsley S, Levy G, Mazzulli T . 1999. Clinical utility of quantitative cytomegalovirus viral load determination for predicting cytomegalovirus disease in liver transplant recipients. Transplantation 68 : 1305 1311[CrossRef].[PubMed]
59. Hirsch HH, Lautenschlager I, Pinsky BA, Cardeñoso L, Aslam S, Cobb B, Vilchez RA, Valsamakis A . 2013. An international multicenter performance analysis of cytomegalovirus load tests. Clin Infect Dis 56 : 367 373[CrossRef].[PubMed]
60. Costa C, Mantovani S, Balloco C, Sidoti F, Fop F, Cavallo R . 2013. Comparison of two nucleic acid extraction and testing systems for HCMV-DNA detection and quantitation on whole blood specimens from transplant patients. J Virol Methods 193 : 579 582[CrossRef].[PubMed]
61. Emery VC, Sabin CA, Cope AV, Gor D, Hassan-Walker AF, Griffiths PD . 2000. Application of viral-load kinetics to identify patients who develop cytomegalovirus disease after transplantation. Lancet 355 : 2032 2036[CrossRef].[PubMed]
62. Kraft CS, Armstrong WS, Caliendo AM . 2012. Interpreting quantitative cytomegalovirus DNA testing: understanding the laboratory perspective. Clin Infect Dis 54 : 1793 1797[CrossRef].[PubMed]
63. Holman CJ, Karger AB, Mullan BD, Brundage RC, Balfour HH Jr . 2012. Quantitative Epstein-Barr virus shedding and its correlation with the risk of post-transplant lymphoproliferative disorder. Clin Transplant 26 : 741 747[CrossRef].[PubMed]
64. Cockfield SM . 2001. Identifying the patient at risk for post-transplant lymphoproliferative disorder. Transpl Infect Dis 3 : 70 78[CrossRef].[PubMed]
65. Gulley ML, Tang W . 2008. Laboratory assays for Epstein-Barr virus-related disease. J Mol Diagn 10 : 279 292[CrossRef].[PubMed]
66. Bakker NA, Verschuuren EA, Veeger NJ, van der Bij W, van Imhoff GW, Kallenberg CG, Hepkema BG . 2008. Quantification of Epstein-Barr virus-DNA load in lung transplant recipients: a comparison of plasma versus whole blood. J Heart Lung Transplant 27 : 7 10[CrossRef].[PubMed]
67. Kinch A, Oberg G, Arvidson J, Falk KI, Linde A, Pauksens K . 2007. Post-transplant lymphoproliferative disease and other Epstein-Barr virus diseases in allogeneic haematopoietic stem cell transplantation after introduction of monitoring of viral load by polymerase chain reaction. Scand J Infect Dis 39 : 235 244[CrossRef].[PubMed]
68. Meerbach A, Wutzler P, Häfer R, Zintl F, Gruhn B . 2008. Monitoring of Epstein-Barr virus load after hematopoietic stem cell transplantation for early intervention in post-transplant lymphoproliferative disease. J Med Virol 80 : 441 454[CrossRef].[PubMed]
69. Toyoda M, Moudgil A, Warady BA, Puliyanda DP, Jordan SC . 2008. Clinical significance of peripheral blood Epstein-Barr viral load monitoring using polymerase chain reaction in renal transplant recipients. Pediatr Transplant 12 : 778 784[CrossRef].[PubMed]
70. Aalto SM, Juvonen E, Tarkkanen J, Volin L, Haario H, Ruutu T, Hedman K . 2007. Epstein-Barr viral load and disease prediction in a large cohort of allogeneic stem cell transplant recipients. Clin Infect Dis 45 : 1305 1309[CrossRef].[PubMed]
71. Greenfield HM, Gharib MI, Turner AJ, Guiver M, Carr T, Will AM, Wynn RF . 2006. The impact of monitoring Epstein-Barr virus PCR in paediatric bone marrow transplant patients: can it successfully predict outcome and guide intervention? Pediatr Blood Cancer 47 : 200 205[CrossRef].[PubMed]
72. Bingler MA, Feingold B, Miller SA, Quivers E, Michaels MG, Green M, Wadowsky RM, Rowe DT, Webber SA . 2008. Chronic high Epstein-Barr viral load state and risk for late-onset posttransplant lymphoproliferative disease/lymphoma in children. Am J Transplant 8 : 442 445[CrossRef].[PubMed]
73. Green M, Soltys K, Rowe DT, Webber SA, Mazareigos G . 2009. Chronic high Epstein-Barr viral load carriage in pediatric liver transplant recipients. Pediatr Transplant 13 : 319 323[CrossRef].[PubMed]
74. Germi R, Lupo J, Semenova T, Larrat S, Magnat N, Grossi L, Seigneurin JM, Morand P . 2012. Comparison of commercial extraction systems and PCR assays for quantification of Epstein-Barr virus DNA load in whole blood. J Clin Microbiol 50 : 1384 1389[CrossRef].[PubMed]
75. Ito Y, Takakura S, Ichiyama S, Ueda M, Ando Y, Matsuda K, Hidaka E, Nakatani K, Nishioka J, Nobori T, Kajiyama N, Kimura H . 2010. Multicenter evaluation of prototype real-time PCR assays for Epstein-Barr virus and cytomegalovirus DNA in whole blood samples from transplant recipients. Microbiol Immunol 54 : 516 522[CrossRef].[PubMed]
76. Fafi-Kremer S, Morand P, Barranger C, Barguès G, Magro S, Bés J, Bourgeois P, Joannes M, Seigneurin JM . 2008. Evaluation of the Epstein-Barr virus R-gene quantification kit in whole blood with different extraction methods and PCR platforms. J Mol Diagn 10 : 78 84[CrossRef].[PubMed]
77. Ruiz G, Peña P, de Ory F, Echevarría JE . 2005. Comparison of commercial real-time PCR assays for quantification of Epstein-Barr virus DNA. J Clin Microbiol 43 : 2053 2057[CrossRef].[PubMed]
78. Cassina G, Russo D, De Battista D, Broccolo F, Lusso P, Malnati MS . 2013. Calibrated real-time polymerase chain reaction for specific quantitation of HHV-6A and HHV-6B in clinical samples. J Virol Methods 189 : 172 179[CrossRef].[PubMed]
79. Costa C, Curtoni A, Bergallo M, Solidoro P, Lorusso M, Delsedime L, Sapino A, Cavallo R . 2011. Quantitative detection of HHV-6 and HHV-7 in transbronchial biopsies from lung transplant recipients. New Microbiol 34 : 275 280.[PubMed]
80. Karlsson T, Mannonen L, Loginov R, Lappalainen M, Höckerstedt K, Lautenschlager I . 2012. Development of a new quantitative real-time HHV-6-PCR and monitoring of HHV-6 DNAaemia after liver transplantation. J Virol Methods 181 : 25 36[CrossRef].[PubMed]
81. Gravel A, Sinnett D, Flamand L . 2013. Frequency of chromosomally-integrated human herpesvirus 6 in children with acute lymphoblastic leukemia. PLoS One 8 : e84322[CrossRef].[PubMed]
82. Sedlak RH, Cook L, Huang ML, Magaret A, Zerr DM, Boeckh M, Jerome KR . 2014. Identification of chromosomally integrated human herpesvirus 6 by droplet digital PCR. Clin Chem 60 : 765 772[CrossRef].[PubMed]
83. Lee SO, Brown RA, Razonable RR . 2011. Clinical significance of pretransplant chromosomally integrated human herpesvirus-6 in liver transplant recipients. Transplantation 92 : 224 229[CrossRef].[PubMed]
84. Espy MJ, Uhl JR, Mitchell PS, Thorvilson JN, Svien KA, Wold AD, Smith TF . 2000. Diagnosis of herpes simplex virus infections in the clinical laboratory by LightCycler PCR. J Clin Microbiol 38 : 795 799.[PubMed]
85. Tan TY, Zou H, Ong DC, Ker KJ, Chio MT, Teo RY, Koh MJ . 2013. Development and clinical validation of a multiplex real-time PCR assay for herpes simplex and varicella zoster virus. Diagn Mol Pathol 22 : 245 248[CrossRef].[PubMed]
86. Bennett S, Carman WF, Gunson RN . 2013. The development of a multiplex real-time PCR for the detection of herpes simplex virus 1 and 2, varizella zoster virus, adenovirus and Chlamydia trachomatis from eye swabs. J Virol Methods 189 : 143 147[CrossRef].[PubMed]
87. Gitman MR, Ferguson D, Landry ML . 2013. Comparison of Simplexa HSV 1 & 2 PCR with culture, immunofluorescence, and laboratory-developed TaqMan PCR for detection of herpes simplex virus in swab specimens. J Clin Microbiol 51 : 3765 3769[CrossRef].[PubMed]
88. Servant A, Laperche S, Lallemand F, Marinho V, De Saint Maur G, Meritet JF, Garbarg-Chenon A . 2002. Genetic diversity within human erythroviruses: identification of three genotypes. J Virol 76 : 9124 9134[CrossRef].[PubMed]
89. Brown KE, Anderson SM, Young NS . 1993. Erythrocyte P antigen: cellular receptor for B19 parvovirus. Science 262 : 114 117[CrossRef].[PubMed]
90. Neild G, Anderson M, Hawes S, Colvin BT . 1986. Parvovirus infection after renal transplant. Lancet 2 : 1226 1227[CrossRef].[PubMed]
91. Eid AJ, Brown RA, Patel R, Razonable RR . 2006. Parvovirus B19 infection after transplantation: a review of 98 cases. Clin Infect Dis 43 : 40 48[CrossRef].[PubMed]
92. Cavallo R, Merlino C, Re D, Bollero C, Bergallo M, Lembo D, Musso T, Leonardi G, Segoloni GP, Ponzi AN . 2003. B19 virus infection in renal transplant recipients. J Clin Virol 26 : 361 368[CrossRef].[PubMed]
93. Ki CS, Kim IS, Kim JW, Lee NY, Kim SH, Lee KW, Kim SJ, Joh JW, Huh WS, Oh HY . 2005. Incidence and clinical significance of human parvovirus B19 infection in kidney transplant recipients. Clin Transplant 19 : 751 755[CrossRef].[PubMed]
94. Zolnourian ZR, Curran MD, Rima BK, Coyle PV, O'Neill HJ, Middleton D . 2000. Parvovirus B19 in kidney transplant patients. Transplantation 69 : 2198 2202[CrossRef].[PubMed]
95. Jordan J, Tiangco B, Kiss J, Koch W . 1998. Human parvovirus B19: prevalence of viral DNA in volunteer blood donors and clinical outcomes of transfusion recipients. Vox Sang 75 : 97 102[CrossRef].[PubMed]
96. Hokynar K, Brunstein J, Söderlund-Venermo M, Kiviluoto O, Partio EK, Konttinen Y, Hedman K . 2000. Integrity and full coding sequence of B19 virus DNA persisting in human synovial tissue. J Gen Virol 81 : 1017 1025[CrossRef].[PubMed]
97. Liefeldt L, Plentz A, Klempa B, Kershaw O, Endres AS, Raab U, Neumayer HH, Meisel H, Modrow S . 2005. Recurrent high level parvovirus B19/genotype 2 viremia in a renal transplant recipient analyzed by real-time PCR for simultaneous detection of genotypes 1 to 3. J Med Virol 75 : 161 169[CrossRef].[PubMed]
98. Aberham C, Pendl C, Gross P, Zerlauth G, Gessner M . 2001. A quantitative, internally controlled real-time PCR Assay for the detection of parvovirus B19 DNA. J Virol Methods 92 : 183 191[CrossRef].[PubMed]
99. Harder TC, Hufnagel M, Zahn K, Beutel K, Schmitt HJ, Ullmann U, Rautenberg P . 2001. New LightCycler PCR for rapid and sensitive quantification of parvovirus B19 DNA guides therapeutic decision-making in relapsing infections. J Clin Microbiol 39 : 4413 4419[CrossRef].[PubMed]
100. Buller RS, Storch G . 2004. Evaluation of a real-time PCR assay using the LightCycler system for detection of parvovirus B19 DNA. J Clin Microbiol 42 : 3326 3328[CrossRef].[PubMed]
101. Arness T, Pedersen R, Dierkhising R, Kremers W, Patel R . 2008. Varicella zoster virus-associated disease in adult kidney transplant recipients: incidence and risk-factor analysis. Transpl Infect Dis 10 : 260 268[CrossRef].[PubMed]
102. Kim DH, Messner H, Minden M, Gupta V, Kuruvilla J, Wright J, Lipton J . 2008. Factors influencing varicella zoster virus infection after allogeneic peripheral blood stem cell transplantation: low-dose acyclovir prophylaxis and pre-transplant diagnosis of lymphoproliferative disorders. Transpl Infect Dis 10 : 90 98[CrossRef].[PubMed]
103. Weinstock DM, Boeckh M, Sepkowitz KA . 2006. Postexposure prophylaxis against varicella zoster virus infection among hematopoietic stem cell transplant recipients. Biol Blood Marrow Transplant 12 : 1096 1097[CrossRef].[PubMed]
104. Espy MJ, Teo R, Ross TK, Svien KA, Wold AD, Uhl JR, Smith TF . 2000. Diagnosis of varicella-zoster virus infections in the clinical laboratory by LightCycler PCR. J Clin Microbiol 38 : 3187 3189.[PubMed]
105. Khare R, Espy MJ, Mandrekar JN, Binnicker MJ . 2014. Comparison of a transplant multiplex viral panel on the ICEPlex system with real-time PCR for detection of cytomegalovirus, Epstein-Barr virus, and BK virus in clinical specimens. J Clin Microbiol 52 : 1711 1713[CrossRef].[PubMed]
106. Popowitch EB, O'Neill SS, Miller MB . 2013. Comparison of the Biofire FilmArray RP, Genmark eSensor RVP, Luminex xTAG RVPv1, and Luminex xTAG RVP fast multiplex assays for detection of respiratory viruses. J Clin Microbiol 51 : 1528 1533[CrossRef].[PubMed]
107. Selvaraju SB, Wurst M, Horvat RT, Selvarangan R . 2009. Evaluation of three analyte-specific reagents for detection and typing of herpes simplex virus in cerebrospinal fluid. Diagn Microbiol Infect Dis 63 : 286 291[CrossRef].[PubMed]
108. Grabarczyk P, Kalińska A, Kara M, Wieczorek R, Ejduk A, Sulkowska E, Gołębiowska-Staroszczyk S, Matysiak M, Baylis SA, Brojer E . 2011. Identification and characterization of acute infection with parvovirus B19 genotype 2 in immunocompromised patients in Poland. J Med Virol 83 : 142 149[CrossRef].[PubMed]
109. Candotti D, Etiz N, Parsyan A, Allain JP . 2004. Identification and characterization of persistent human erythrovirus infection in blood donor samples. J Virol 78 : 12169 12178[CrossRef].[PubMed]


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Examples of PCR assays for the detection and identification of viruses impacting transplant recipients

Citation: Binnicker M, Razonable R. 2016. Molecular Diagnostics for Viral Infections in Transplant Recipients, p 476-486. In Persing D, Tenover F, Hayden R, Ieven M, Miller M, Nolte F, Tang Y, van Belkum A (ed), Molecular Microbiology. ASM Press, Washington, DC. doi: 10.1128/9781555819071.ch34

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