Chapter 37 : Molecular Approaches for Diagnosis of Malaria and the Characterization of Genetic Markers for Drug Resistance

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Human malaria is caused by infection with protozoan parasites of the genus Four species of are well known to cause malaria in humans: , , , and (with two subspecies, and [1]). Recently, a fifth species has been added to the list: the simian malaria species has been discovered to be a major cause of zoonotic human malaria in parts of Southeast Asia (2). The parasite species vary in the severity of disease they cause, as a result of differences in their biology, with the highest morbidity and mortality resulting from infection with . Correct identification of the infecting species is required to select appropriate and effective antimalarial drugs for treatment.

Citation: Ranford-Cartwright L, Ciuffreda L. 2016. Molecular Approaches for Diagnosis of Malaria and the Characterization of Genetic Markers for Drug Resistance, p 516-529. In Persing D, Tenover F, Hayden R, Ieven M, Miller M, Nolte F, Tang Y, van Belkum A (ed), Molecular Microbiology. ASM Press, Washington, DC. doi: 10.1128/9781555819071.ch37
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Image of FIGURE 1

(A) Thick and (B) thin blood films showing ring forms. (Images taken from the Public Health Image Library, Centers for Disease Control and Prevention, courtesy CDC/Dr. Mae Melvin [public domain image]).

Citation: Ranford-Cartwright L, Ciuffreda L. 2016. Molecular Approaches for Diagnosis of Malaria and the Characterization of Genetic Markers for Drug Resistance, p 516-529. In Persing D, Tenover F, Hayden R, Ieven M, Miller M, Nolte F, Tang Y, van Belkum A (ed), Molecular Microbiology. ASM Press, Washington, DC. doi: 10.1128/9781555819071.ch37
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Image of FIGURE 2

NASBA. Reprinted from ( ) with permission of the publisher.

Citation: Ranford-Cartwright L, Ciuffreda L. 2016. Molecular Approaches for Diagnosis of Malaria and the Characterization of Genetic Markers for Drug Resistance, p 516-529. In Persing D, Tenover F, Hayden R, Ieven M, Miller M, Nolte F, Tang Y, van Belkum A (ed), Molecular Microbiology. ASM Press, Washington, DC. doi: 10.1128/9781555819071.ch37
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Image of FIGURE 3

Schematic representation of the mechanism of LAMP. Reprinted from ( ) with permission of the publisher.

Citation: Ranford-Cartwright L, Ciuffreda L. 2016. Molecular Approaches for Diagnosis of Malaria and the Characterization of Genetic Markers for Drug Resistance, p 516-529. In Persing D, Tenover F, Hayden R, Ieven M, Miller M, Nolte F, Tang Y, van Belkum A (ed), Molecular Microbiology. ASM Press, Washington, DC. doi: 10.1128/9781555819071.ch37
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