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Category: Clinical Microbiology
Molecular Detection of Gastrointestinal Parasites, Page 1 of 2
< Previous page | Next page > /docserver/preview/fulltext/10.1128/9781555819071/9781555819088.ch38-1.gif /docserver/preview/fulltext/10.1128/9781555819071/9781555819088.ch38-2.gifAbstract:
In parasitology, the importance of DNA-based testing methods became apparent in the 1990s following the redescription of the potentially invasive Entamoeba histolytica and the nonpathogenic Entamoeba dispar (1). Since these two species are morphologically indistinguishable, nucleic acid-based detection methods were introduced in the clinical laboratory for their differentiation in cases where microscopy lacked specificity. In a similar way, molecular applications gradually replaced tests in which microscopy was time-consuming or inconclusive, for example, in the detection of microsporidia (2). When real-time PCR was introduced, the idea of using multiplex PCR assays for the simultaneous detection of various pathogens as first-line diagnosis gradually developed (3).
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