Chapter 38 : Chlamydiae

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The are a family of small, metabolically dependent bacteria with a unique intracellular life cycle (1). As a result of the obligate intracellular growth of these organisms in eukaryotic cells, they are handled in ways more closely resembling the detection of viruses than bacteria. Thus, inclusion of these organisms in this manual is appropriate.

Citation: Van Der Pol B, Gaydos C. 2016. Chlamydiae, p 545-559. In Loeffelholz M, Hodinka R, Young S, Pinsky B (ed), Clinical Virology Manual, Fifth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555819156.ch38
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1. Hatch TP, Allan I, Pearce JH . 1984. Structural and polypeptide differences between envelopes of infective and reproductive life cycle forms of Chlamydia spp. J Bacteriol 157 : 13 20.[PubMed]
2. Everett KDE, Bush RM, Andersen AA . 1999. Emended description of the order Chlamydiales, proposal of Parachlamydiaceae fam. nov. and Simkaniaceae fam. nov., each containing one monotypic genus, revised taxonomy of the family Chlamydiaceae, including a new genus and five new species, and standards for the identification of organisms. Int J Syst Bacteriol 49 : 415 440[CrossRef].[PubMed]
3. Stephens RS, Myers G, Eppinger M, Bavoil PM . 2009. Divergence without difference: phylogenetics and taxonomy of Chlamydia resolved. FEMS Immunol Med Microbiol 55 : 115 119[CrossRef].[PubMed]
4. Stephens RS, Kalman S, Lammel C, Fan J, Marathe R, Aravind L, Mitchell W, Olinger L, Tatusov RL, Zhao Q, Koonin EV, Davis RW . 1998. Genome sequence of an obligate intracellular pathogen of humans: chlamydia trachomatis. Science 282 : 754 759[CrossRef].[PubMed]
5. Greub G . 2013. International Committee on Systematics of Prokaryotes Subcommittee on the taxonomy of Chlamydiae: minutes of the closed meeting, 23 February 2011, Ascona, Switzerland. Int J Syst Evol Microbiol 63 : 1934 1935[CrossRef].
6. Bachmann NL, Polkinghorne A, Timms P . 2014. Chlamydia genomics: providing novel insights into chlamydial biology. Trends Microbiol 22 : 464 472[CrossRef].[PubMed]
7. Fields KA, Hackstadt T . 2002. The chlamydial inclusion: escape from the endocytic pathway. Annu Rev Cell Dev Biol 18 : 221 245[CrossRef].[PubMed]
8. Hackstadt T, Scidmore-Carlson MA, Shaw EI, Fischer ER . 1999. The Chlamydia trachomatis IncA protein is required for homotypic vesicle fusion. Cell Microbiol 1 : 119 130[CrossRef].[PubMed]
9. Geisler WM, Suchland RJ, Rockey DD, Stamm WE . 2001. Epidemiology and clinical manifestations of unique Chlamydia trachomatis isolates that occupy nonfusogenic inclusions. J Infect Dis 184 : 879 884[CrossRef].[PubMed]
10. Millman K, Black CM, Johnson RE, Stamm WE, Jones RB, Hook EW, Martin DH, Bolan G, Tavaré S, Dean D . 2004. Population-based genetic and evolutionary analysis of Chlamydia trachomatis urogenital strain variation in the United States. J Bacteriol 186 : 2457 2465[CrossRef].[PubMed]
11. Won H, Ramachandran P, Steece R, Van Der Pol B, Moncada J, Schachter J, Gaydos C . 2013. Is there evidence of the new variant Chlamydia trachomatis in the United States? Sex Transm Dis 40 : 352 353[CrossRef].[PubMed]
12. Unemo M, Clarke IN . 2011. The Swedish new variant of Chlamydia trachomatis. Curr Opin Infect Dis 24 : 62 69[CrossRef].[PubMed]
13. Unemo M, Seth-Smith HM, Cutcliffe LT, Skilton RJ, Barlow D, Goulding D, Persson K, Harris SR, Kelly A, Bjartling C, Fredlund H, Olcén P, Thomson NR, Clarke IN . 2010. The Swedish new variant of Chlamydia trachomatis: genome sequence, morphology, cell tropism and phenotypic characterization. Microbiology 156 : 1394 1404[CrossRef].[PubMed]
14. Grayston JT, Kuo C-C, Campbell LA, Wang S-P . 1989. Chlamydia pneumoniae sp. nov. for Chlamydia sp. strain TWAR. Int J Syst Bacteriol 39 : 88 90[CrossRef].
15. Gaydos CA, Palmer L, Quinn TC, Falkow S, Eiden JJ . 1993. Phylogenetic relationship of Chlamydia pneumoniae to Chlamydia psittaci and Chlamydia trachomatis as determined by analysis of 16S ribosomal DNA sequences. Int J Syst Bacteriol 43 : 610 612[CrossRef].[PubMed]
16. Brunham RC, Rey-Ladino J . 2005. Immunology of Chlamydia infection: implications for a Chlamydia trachomatis vaccine. Nat Rev Immunol 5 : 149 161[CrossRef].[PubMed]
17. Beatty WL, Byrne GI, Morrison RP . 1993. Morphologic and antigenic characterization of interferon gamma-mediated persistent Chlamydia trachomatis infection in vitro. Proc Natl Acad Sci USA 90 : 3998 4002[CrossRef].[PubMed]
18. Dean D, Suchland RJ, Stamm WE . 2000. Evidence for long-term cervical persistence of Chlamydia trachomatis by omp1 genotyping. J Infect Dis 182 : 909 916[CrossRef].[PubMed]
19. World Health Organization . 2012. Global incidence and prevalence of selected curable sexually transmitted infections-2008. WHO Press.
20. Centers for Disease Control and Prevention . 2012. Sexually Transmitted. Dis Surveill : 2011. U.S. Department of Health and Human Services.
21. Torrone E, Papp J, Weinstock H Centers for Disease Control and Prevention (CDC) . 2014. Prevalence of Chlamydia trachomatis genital infection among persons aged 14–39 years—United States, 2007–2012. MMWR Morb Mortal Wkly Rep 63 : 834 838.[PubMed]
22. Adams DA, Jajosky RA, Ajani U, Kriseman J, Sharp P, Onwen DH, Schley AW, Anderson WJ, Grigoryan A, Aranas AE, Wodajo MS, Abellera JP Centers for Disease Control and Prevention (CDC) . 2014. Summary of notifiable diseases—United States, 2012. MMWR Morb Mortal Wkly Rep 61 : 1 121.[PubMed]
23. Burton MJ, Mabey DCW . 2009. The global burden of trachoma: a review. PLoS Negl Trop Dis 3 : e460[CrossRef].[PubMed]
24. World Health Organization . 2007. Global Initiative for the Elimination of Avoidable Blindness: action plan 2006–2011.
25. World Health Organization . 2003. Guidelines for the management of Sexually Transmitted Infections. WHO Press.
26. Detels R, Green AM, Klausner JD, Katzenstein D, Gaydos C, Handsfield H, Pequegnat W, Mayer K, Hartwell TD, Quinn TC . 2011. The incidence and correlates of symptomatic and asymptomatic Chlamydia trachomatis and Neisseria gonorrhoeae infections in selected populations in five countries. Sex Transm Dis 38 : 503 509.[PubMed]
27. Nieuwenhuis RF, Ossewaarde JM, Götz HM, Dees J, Thio HB, Thomeer MGJ, den Hollander JC, Neumann MHA, van der Meijden WI . 2004. Resurgence of lymphogranuloma venereum in Western Europe: an outbreak of Chlamydia trachomatis serovar l2 proctitis in The Netherlands among men who have sex with men. Clin Infect Dis 39 : 996 1003[CrossRef].[PubMed]
28. Price MJ, Ades AE, De Angelis D, Welton NJ, Macleod J, Soldan K, Simms I, Turner K, Horner PJ . 2013. Risk of pelvic inflammatory disease following Chlamydia trachomatis infection: analysis of prospective studies with a multistate model. Am J Epidemiol 178 : 484 492[CrossRef].[PubMed]
29. Bender N, Herrmann B, Andersen B, Hocking JS, van Bergen J, Morgan J, van den Broek IV, Zwahlen M, Low N . 2011. Chlamydia infection, pelvic inflammatory disease, ectopic pregnancy and infertility: cross-national study. Sex Transm Infect 87 : 601 608[CrossRef].[PubMed]
30. French CE, Hughes G, Nicholson A, Yung M, Ross JD, Williams T, Soldan K . 2011. Estimation of the rate of pelvic inflammatory disease diagnoses: Trends in England, 2000–2008. Sex. Transm. Dis. 38: 158 162.
31. Rekart ML, Gilbert M, Meza R, Kim PH, Chang M, Money DM, Brunham RC . 2013. Chlamydia public health programs and the epidemiology of pelvic inflammatory disease and ectopic pregnancy. J Infect Dis 207 : 30 38[CrossRef].[PubMed]
32. Tuffrey M, Alexander F, Inman C, Ward ME . 1990. Correlation of infertility with altered tubal morphology and function in mice with salpingitis induced by a human genital-tract isolate of Chlamydia trachomatis. J Reprod Fertil 88 : 295 305[CrossRef].[PubMed]
33. Patton DL, Askienazy-Elbhar M, Henry-Suchet J, Campbell LA, Cappuccio A, Tannous W, Wang SP, Kuo CC . 1994. Detection of Chlamydia trachomatis in fallopian tube tissue in women with postinfectious tubal infertility. Am J Obstet Gynecol 171 : 95 101[CrossRef].[PubMed]
34. Hammerschlag MR, Kohlhoff SA, Gaydos CA, . 2014. Chlamydia pneumoniae, p. 2174 2182. In Mandell GL, Bennett JE, Dolin R, Blaser MJ (eds.), Principles and Practice of Infectious Diseases, 8th ed. Elsevier, Sanders Inc., Philadelphia, PA.
35. Cabbage S, Ieronimakis N, Preusch M, Lee A, Ricks J, Janebodin K, Hays A, Wijelath ES, Reyes M, Campbell LA, Rosenfeld ME . 2014. Chlamydia pneumoniae infection of lungs and macrophages indirectly stimulates the phenotypic conversion of smooth muscle cells and mesenchymal stem cells: potential roles in vascular calcification and fibrosis. Pathog Dis 72 : 61 69[CrossRef].[PubMed]
36. Hahn DL, Schure A, Patel K, Childs T, Drizik E, Webley W . 2012. Chlamydia pneumoniae-specific IgE is prevalent in asthma and is associated with disease severity. PLoS One 7 : e35945[CrossRef].[PubMed]
37. Brandt J, Cader A, Semler L, Hammond C, Bell M, Devins M, Smith J, Joseph N, Velez M, Galluzzi K, Balin B, Appelt D . 2014. Electron microscopy studies elucidate morphological forms of Chlamydia pneumoniae in blood samples from patients diagnosed with mild cognitive impairment (MCI) and Alzheimer's disease (AD) (LB81). FASEB J 28 : LB81.
38. Carter JD, Espinoza LR, Inman RD, Sneed KB, Ricca LR, Vasey FB, Valeriano J, Stanich JA, Oszust C, Gerard HC, Hudson AP . 2010. Combination antibiotics as a treatment for chronic Chlamydia-induced reactive arthritis: a double-blind, placebo-controlled, prospective trial. Arthritis Rheum 62 : 1298 1307[CrossRef].[PubMed]
39. Workowski KA, Berman S Centers for Disease Control and Prevention (CDC) . 2010. Sexually transmitted diseases treatment guidelines, 2010. MMWR Recomm Rep 59(RR-12) : 1 110.[PubMed]
40. LeFevre ML . 2014. Screening for Chlamydia and Gonorrhea: U.S. Preventive Services Task Force Recommendation Statement Screening for Chlamydia and Gonorrhea. Ann. Intern. Med. 161( 12): 1 30.
41. Bachmann LH, Johnson RE, Cheng H, Markowitz L, Papp JR, Palella FJ Jr, Hook EW III . 2010. Nucleic acid amplification tests for diagnosis of Neisseria gonorrhoeae and Chlamydia trachomatis rectal infections. J Clin Microbiol 48 : 1827 1832[CrossRef].[PubMed]
42. Harding-Esch EM, Edwards T, Mkocha H, Munoz B, Holland MJ, Burr SE, Sillah A, Gaydos CA, Stare D, Mabey DCW, Bailey RL, West SK PRET Partnership . 2010. Trachoma prevalence and associated risk factors in the gambia and Tanzania: baseline results of a cluster randomised controlled trial. PLoS Negl Trop Dis 4 : e861[CrossRef].[PubMed]
43. West SK, Munoz B, Mkocha H, Gaydos CA, Quinn TC . 2011. Number of years of annual mass treatment with azithromycin needed to control trachoma in hyper-endemic communities in Tanzania. J Infect Dis 204 : 268 273[CrossRef].[PubMed]
44. Cajas-Monson LC, Mkocha H, Muñoz B, Quinn TC, Gaydos CA, West SK . 2011. Risk factors for ocular infection with Chlamydia trachomatis in children 6 months following mass treatment in Tanzania. PLoS Negl Trop Dis 5 : e978[CrossRef].[PubMed]
45. Hammerschlag MR, Kohlhoff SA . 2012. Treatment of chlamydial infections. Expert Opin Pharmacother 13 : 545 552[CrossRef].[PubMed]
46. Moncada J, Schachter J, Liska S, Shayevich C, Klausner JD . 2009. Evaluation of self-collected glans and rectal swabs from men who have sex with men for detection of Chlamydia trachomatis and Neisseria gonorrhoeae by use of nucleic acid amplification tests. J Clin Microbiol 47 : 1657 1662[CrossRef].[PubMed]
47. Leslie DE, Azzato F, Ryan N, Fyfe J . 2003. An assessment of the Roche Amplicor Chlamydia trachomatis/Neisseria gonorrhoeae multiplex PCR assay in routine diagnostic use on a variety of specimen types. Commun Dis Intell Q Rep 27 : 373 379.[PubMed]
48. Pittaras TE, Papaparaskevas J, Houhoula DP, Legakis NJ, Frangouli E, Katsambas A, Tsakris A, Papadogeorgakis H . 2008. Comparison of penile skin swab with intra-urethral swab and first void urine for polymerase chain reaction-based diagnosis of Chlamydia trachomatis urethritis in male patients. Sex Transm Dis 35 : 999 1001.[PubMed]
49. Dize L, Agreda P, Quinn N, Barnes MR, Hsieh Y-H, Gaydos CA . 2013. Comparison of self-obtained penile-meatal swabs to urine for the detection of C. trachomatis, N. gonorrhoeae and T. vaginalis. Sex Transm Infect 89 : 305 307[CrossRef].[PubMed]
50. Kumamoto Y, Matsumoto T, Fujisawa M, Arakawa S . 2012. Detection of Chlamydia trachomatis and Neisseria gonorrhoeae in urogenital and oral specimens using the cobas® 4800, APTIMA Combo 2® TMA, and ProbeTec™ ET SDA assays. Eur J Microbiol Immunol (Bp) 2 : 121 127[CrossRef].[PubMed]
51. Schachter J, Moncada J, Liska S, Shayevich C, Klausner JD . 2008. Nucleic acid amplification tests in the diagnosis of chlamydial and gonococcal infections of the oropharynx and rectum in men who have sex with men. Sex Transm Dis 35 : 637 642[CrossRef].[PubMed]
52. Ota KV, Tamari IE, Smieja M, Jamieson F, Jones KE, Towns L, Juzkiw J, Richardson SE . 2009. Detection of Neisseria gonorrhoeae and Chlamydia trachomatis in pharyngeal and rectal specimens using the BD Probetec ET system, the Gen-Probe Aptima Combo 2 assay and culture. Sex Transm Infect 85 : 182 186[CrossRef].[PubMed]
53. Peters RP, Verweij SP, Nijsten N, Ouburg S, Mutsaers J, Jansen CL, van Leeuwen AP, Morré SA . 2011. Evaluation of sexual history-based screening of anatomic sites for chlamydia trachomatis and neisseria gonorrhoeae infection in men having sex with men in routine practice. BMC Infect Dis 11 : 203[CrossRef].[PubMed]
54. Bax CJ, Quint KD, Peters RP, Ouburg S, Oostvogel PM, Mutsaers JA, Dörr PJ, Schmidt S, Jansen C, van Leeuwen AP, Quint WG, Trimbos JB, Meijer CJ, Morré SA . 2011. Analyses of multiple-site and concurrent Chlamydia trachomatis serovar infections, and serovar tissue tropism for urogenital versus rectal specimens in male and female patients. Sex Transm Infect 87 : 503 507[CrossRef].[PubMed]
55. Marcus JL, Bernstein KT, Stephens SC, Snell A, Kohn RP, Liska S, Klausner JD . 2010. Sentinel surveillance of rectal chlamydia and gonorrhea among males—San Francisco, 2005–2008. Sex Transm Dis 37 : 59 61[CrossRef].[PubMed]
56. Hunte T, Alcaide M, Castro J . 2010. Rectal infections with chlamydia and gonorrhoea in women attending a multiethnic sexually transmitted diseases urban clinic. Int J STD AIDS 21 : 819 822[CrossRef].[PubMed]
57. van der Helm JJ, Hoebe CJ, van Rooijen MS, Brouwers EE, Fennema HS, Thiesbrummel HF, Dukers-Muijrers NH . 2009. High performance and acceptability of self-collected rectal swabs for diagnosis of Chlamydia trachomatis and Neisseria gonorrhoeae in men who have sex with men and women. Sex Transm Dis 36 : 493 497[CrossRef].[PubMed]
58. Baker J, Plankey M, Josayma Y, Elion R, Chiliade P, Shahkolahi A, Menna M, Miniter K, Slack R, Yang Y, Masterman B, Margolick JB . 2009. The prevalence of rectal, urethral, and pharyngeal Neisseria gonorrheae and Chlamydia trachomatis among asymptomatic men who have sex with men in a prospective cohort in Washington, DC. AIDS Patient Care STDS 23 : 585 588[CrossRef].[PubMed]
59. Alexander S, Ison C, Parry J, Llewellyn C, Wayal S, Richardson D, Phillips A, Smith H, Fisher M Brighton Home Sampling Kits Steering Group . 2008. Self-taken pharyngeal and rectal swabs are appropriate for the detection of Chlamydia trachomatis and Neisseria gonorrhoeae in asymptomatic men who have sex with men. Sex Transm Infect 84 : 488 492[CrossRef].[PubMed]
60. Bachmann LH, Johnson RE, Cheng H, Markowitz L, Papp JR, Palella FJ Jr, Hook EW III . 2010. Nucleic acid amplification tests for diagnosis of Neisseria gonorrhoeae and Chlamydia trachomatis rectal infections. J Clin Microbiol 48 : 1827 1832[CrossRef].[PubMed]
61. Goldenberg SD, Finn J, Sedudzi E, White JA, Tong CYW . 2012. Performance of the GeneXpert CT/NG assay compared to that of the Aptima AC2 assay for detection of rectal Chlamydia trachomatis and Neisseria gonorrhoeae by use of residual Aptima Samples. J Clin Microbiol 50 : 3867 3869[CrossRef].[PubMed]
62. Dodge B, Van Der Pol B, Reece M, Malebranche D, Martinez O, Goncalves G, Schnarrs P, Nix R, Fortenberry JD . 2012. Rectal self-sampling in non-clinical venues for detection of sexually transmissible infections among behaviourally bisexual men. Sex Health 9 : 190 191.[PubMed]
63. Rosenberger JG, Dodge B, Van Der Pol B, Reece M, Herbenick D, Fortenberry JD . 2011. Reactions to self-sampling for ano-rectal sexually transmitted infections among men who have sex with men: a qualitative study. Arch Sex Behav 40 : 281 288[CrossRef].[PubMed]
64. Ladd J, Hsieh Y-H, Barnes M, Quinn N, Jett-Goheen M, Gaydos CA . 2014. Female users of internet-based screening for rectal STIs: descriptive statistics and correlates of positivity. Sex Transm Infect 90 : 485 490[CrossRef].[PubMed]
65. Freeman AH, Bernstein KT, Kohn RP, Philip S, Rauch LM, Klausner JD . 2011. Evaluation of self-collected versus clinician-collected swabs for the detection of Chlamydia trachomatis and Neisseria gonorrhoeae pharyngeal infection among men who have sex with men. Sex Transm Dis 38 : 1036 1039[CrossRef].[PubMed]
66. Dize L, West S, Williams JA, Van Der Pol B, Quinn TC, Gaydos CA . 2013. Comparison of the Abbott m2000 RealTime CT assay and the Cepheid GeneXpert CT/NG assay to the Roche Amplicor CT assay for detection of Chlamydia trachomatis in ocular samples from Tanzania. J Clin Microbiol 51 : 1611 1613[CrossRef].[PubMed]
67. Yang JL, Hong KC, Schachter J, Moncada J, Lekew T, House JI, Zhou Z, Neuwelt MD, Rutar T, Halfpenny C, Shah N, Whitcher JP, Lietman TM . 2009. Detection of Chlamydia trachomatis ocular infection in trachoma-endemic communities by rRNA amplification. Invest Ophthalmol Vis Sci 50 : 90 94[CrossRef].[PubMed]
68. Thomas BJ, Evans RT, Hawkins DA, Taylor-Robinson D . 1984. Sensitivity of detecting Chlamydia trachomatis elementary bodies in smears by use of a fluorescein labelled monoclonal antibody: comparison with conventional chlamydial isolation. J Clin Pathol 37 : 812 816[CrossRef].[PubMed]
69. Stamm WE, Harrison HR, Alexander ER, Cles LD, Spence MR, Quinn TC . 1984. Diagnosis of Chlamydia trachomatis infections by direct immunofluorescence staining of genital secretions. A multicenter trial. Ann Intern Med 101 : 638 641[CrossRef].[PubMed]
70. Beebe JL, Masters H, Jungkind D, Heltzel DM, Weinberg A . 1996. Confirmation of the Syva MicroTrak enzyme immunoassay for chlamydia trachomatis by Syva Direct Fluorescent Antibody Test. Sex Transm Dis 23 : 465 470[CrossRef].[PubMed]
71. Van Der Pol B, Williams J, Smith N, Batteiger B, Cullen A, Edens T, Davis K, Salim H, Chou V, Scearce L, Blutman J, Payne W . 2001 . Comparison of the semi-automated Digene Rapid Capture System TM with the manual system for the Hybrid Capture 2 Chlamydia Assay using endocervical samples. 14th meeting of the International Society for Sexually Transmitted Diseases Research Berlin, Germany.
72. Beltrami JF, Farley TA, Hamrick JT, Cohen DA, Martin DH . 1998. Evaluation of the Gen-Probe PACE 2 assay for the detection of asymptomatic Chlamydia trachomatis and Neisseria gonorrhoeae infections in male arrestees. Sex Transm Dis 25 : 501 504[CrossRef].[PubMed]
73. Darwin LH, Cullen AP, Arthur PM, Long CD, Smith KR, Girdner JL, Hook EW III, Quinn TC, Lorincz AT . 2002. Comparison of Digene hybrid capture 2 and conventional culture for detection of Chlamydia trachomatis and Neisseria gonorrhoeae in cervical specimens. J Clin Microbiol 40 : 641 644[CrossRef].[PubMed]
74. Schachter J, Hook EW III, McCormack WM, Quinn TC, Chernesky M, Chong S, Girdner JI, Dixon PB, DeMeo L, Williams E, Cullen A, Lorincz A . 1999. Ability of the digene hybrid capture II test to identify Chlamydia trachomatis and Neisseria gonorrhoeae in cervical specimens. J Clin Microbiol 37 : 3668 3671.[PubMed]
75. Modarress KJ, Cullen AP, Jaffurs WJ Sr, Troutman GL, Mousavi N, Hubbard RA, Henderson S, Lörincz AT . 1999. Detection of Chlamydia trachomatis and Neisseria gonorrhoeae in swab specimens by the Hybrid Capture II and PACE 2 nucleic acid probe tests. Sex Transm Dis 26 : 303 308[CrossRef].[PubMed]
76. Beebe JL, Sharpton TR, Zanto SN, Steece RS, Rogers C, Mottice SL . 1997. Performance characteristics of the Gen-Probe Probe Competition Assay used as a supplementary test for the Gen-Probe PACE 2 and 2C assays for detection of Chlamydia trachomatis. J Clin Microbiol 35 : 477 478.[PubMed]
77. Newhall WJ, Johnson RE, DeLisle S, Fine D, Hadgu A, Matsuda B, Osmond D, Campbell J, Stamm WE . 1999. Head-to-head evaluation of five chlamydia tests relative to a quality-assured culture standard. J Clin Microbiol 37 : 681 685.[PubMed]
78. Van Der Pol B, Ferrero DV, Buck-Barrington L, Hook E III, Lenderman C, Quinn T, Gaydos CA, Lovchik J, Schachter J, Moncada J, Hall G, Tuohy MJ, Jones RB . 2001. Multicenter evaluation of the BDProbeTec ET System for detection of Chlamydia trachomatis and Neisseria gonorrhoeae in urine specimens, female endocervical swabs, and male urethral swabs. J Clin Microbiol 39 : 1008 1016[CrossRef].[PubMed]
79. Van Der Pol B, Quinn TC, Gaydos CA, Crotchfelt K, Schachter J, Moncada J, Jungkind D, Martin DH, Turner B, Peyton C, Jones RB . 2000. Multicenter evaluation of the AMPLICOR and automated COBAS AMPLICOR CT/NG tests for detection of Chlamydia trachomatis. J Clin Microbiol 38 : 1105 1112.[PubMed]
80. Schachter J, Stamm WE, Quinn TC, Andrews WW, Burczak JD, Lee HH . 1994. Ligase chain reaction to detect Chlamydia trachomatis infection of the cervix. J Clin Microbiol 32 : 2540 2543.[PubMed]
81. Chernesky MA, Lee H, Schachter J, Burczak JD, Stamm WE, McCormack WM, Quinn TC . 1994. Diagnosis of Chlamydia trachomatis urethral infection in symptomatic and asymptomatic men by testing first-void urine in a ligase chain reaction assay. J Infect Dis 170 : 1308 1311[CrossRef].[PubMed]
82. Black CM, Marrazzo J, Johnson RE, Hook EW III, Jones RB, Green TA, Schachter J, Stamm WE, Bolan G, St Louis ME, Martin DH . 2002. Head-to-head multicenter comparison of DNA probe and nucleic acid amplification tests for Chlamydia trachomatis infection in women performed with an improved reference standard. J Clin Microbiol 40 : 3757 3763[CrossRef].[PubMed]
83. Papp J, Schachter J, Gaydos C, Van Der Pol B . 2014. Recommendations for the laboratory-based detection of Chlamydia trachomatis and Neisseria gonorrhoeae-2014. MMWR Morb Mortal Wkly Rep 63 : 1 24.[PubMed]
84. Schachter J, Chow JM, Howard H, Bolan G, Moncada J . 2006. Detection of Chlamydia trachomatis by nucleic acid amplification testing: our evaluation suggests that CDC-recommended approaches for confirmatory testing are ill-advised. J Clin Microbiol 44 : 2512 2517[CrossRef].[PubMed]
85. Scragg S, Bingham A, Mallinson H . 2006. Should Chlamydia trachomatis confirmation make you cross? Performance of collection kits tested across three nucleic acid amplification test platforms. Sex Transm Infect 82 : 295 297[CrossRef].[PubMed]
86. Chow JM, Bauer HM, Bolan G . 2012. Repeating low-positive nucleic acid amplification test results for Chlamydia trachomatis and Neisseria gonorrhoeae: assessment of current practice in selected california public- and private-sector laboratories. J Clin Microbiol 50 : 539[CrossRef].[PubMed]
87. Van Der Pol B, Body B, Nye M, Eisenhut C, Taylor S, Liesenfeld O . 2011. Vaginal swabs are the optimal sample for screening women for chlamydia and gonorrhea infection using the Roche cobas 4800 system. 21st Meeting of the European Congress on Clinical Microbiology and Infectious Diseases.
88. Taylor S, Lillis R, Body B, Nye M, Williams J, Van Der Pol B . 2011. Evaluation of the Roche cobas 4800 for detection of Chlamydia trachomatis and Neisseria gonorrhoeae in men. 19th Biennial meeting of the International Society for STD Research Quebec City, CAN.
89. Rockett R, Goire N, Limnios A, Turra M, Higgens G, Lambert SB, Bletchly C, Nissen MD, Sloots TP, Whiley DM . 2010. Evaluation of the cobas 4800 CT/NG test for detecting Chlamydia trachomatis and Neisseria gonorrhoeae. Sex Transm Infect 86 : 470 473[CrossRef].[PubMed]
90. Van Der Pol B, Liesenfeld O, Williams JA, Taylor SN, Lillis RA, Body BA, Nye M, Eisenhut C, Hook EW III . 2012. Performance of the cobas CT/NG test compared to the Aptima AC2 and Viper CTQ/GCQ assays for detection of Chlamydia trachomatis and Neisseria gonorrhoeae. J Clin Microbiol 50 : 2244 2249[CrossRef].[PubMed]
91. Møller JK, Pedersen LN, Persson K . 2010. Comparison of the Abbott RealTime CT new formulation assay with two other commercial assays for detection of wild-type and new variant strains of Chlamydia trachomatis. J Clin Microbiol 48 : 440 443[CrossRef].[PubMed]
92. Levett PN, Brandt K, Olenius K, Brown C, Montgomery K, Horsman GB . 2008. Evaluation of three automated nucleic acid amplification systems for detection of Chlamydia trachomatis and Neisseria gonorrhoeae in first-void urine specimens. J Clin Microbiol 46 : 2109 2111[CrossRef].[PubMed]
93. Marshall R, Chernesky M, Jang D, Hook EW, Cartwright CP, Howell-Adams B, Ho S, Welk J, Lai-Zhang J, Brashear J, Diedrich B, Otis K, Webb E, Robinson J, Yu H . 2007. Characteristics of the m2000 automated sample preparation and multiplex real-time PCR system for detection of Chlamydia trachomatis and Neisseria gonorrhoeae. J Clin Microbiol 45 : 747 751[CrossRef].[PubMed]
94. Gaydos CA, Van Der Pol B, Jett-Goheen M, Barnes M, Quinn N, Clark C, Daniel GE, Dixon PB, Hook EW III CT/NG Study Group . 2013. Performance of the Cepheid CT/NG Xpert Rapid PCR test for detection of Chlamydia trachomatis and Neisseria gonorrhoeae. J Clin Microbiol 51 : 1666 1672[CrossRef].[PubMed]
95. Tabrizi SN, Unemo M, Golparian D, Twin J, Limnios AE, Lahra M, Guy R TTANGO Investigators . 2013. Analytical evaluation of GeneXpert CT/NG, the first genetic point-of-care assay for simultaneous detection of Neisseria gonorrhoeae and Chlamydia trachomatis. J Clin Microbiol 51 : 1945 1947[CrossRef].[PubMed]
96. Gaydos CA . 2014. Review of use of a new rapid real-time PCR, the Cepheid GeneXpert® (Xpert) CT/NG assay, for Chlamydia trachomatis and Neisseria gonorrhoeae: results for patients while in a clinical setting. Expert Rev Mol Diagn 14 : 135 137[CrossRef].[PubMed]
97. Taylor SN, Van Der Pol B, Lillis R, Hook EW III, Lebar W, Davis T, Fuller D, Mena L, Fine P, Gaydos CA, Martin DH . 2011. Clinical evaluation of the BD ProbeTec™ Chlamydia trachomatis Qx amplified DNA assay on the BD Viper™ system with XTR™ technology. Sex Transm Dis 38 : 603 609[CrossRef].[PubMed]
98. Lowe P, O'Loughlin P, Evans K, White M, Bartley PB, Vohra R . 2006. Comparison of the Gen-Probe APTIMA Combo 2 assay to the AMPLICOR CT/NG assay for detection of Chlamydia trachomatis and Neisseria gonorrhoeae in urine samples from Australian men and women. J Clin Microbiol 44 : 2619 2621[CrossRef].[PubMed]
99. Boyadzhyan B, Yashina T, Yatabe JH, Patnaik M, Hill CS . 2004. Comparison of the APTIMA CT and GC assays with the APTIMA combo 2 assay, the Abbott LCx assay, and direct fluorescent-antibody and culture assays for detection of Chlamydia trachomatis and Neisseria gonorrhoeae. J Clin Microbiol 42 : 3089 3093[CrossRef].[PubMed]
100. Gaydos CA, Quinn TC, Willis D, Weissfeld A, Hook EW, Martin DH, Ferrero DV, Schachter J . 2003. Performance of the APTIMA Combo 2 assay for detection of Chlamydia trachomatis and Neisseria gonorrhoeae in female urine and endocervical swab specimens. J Clin Microbiol 41 : 304 309[CrossRef].[PubMed]
101. Lewis N, Dube G, Carter C, Pitt R, Alexander S, Ison CA, Harding J, Brown L, Fryer J, Hodson J, Ross J . 2012. Chlamydia and gonorrhoea contamination of clinic surfaces. Sex Transm Infect 88 : 418 421[CrossRef].[PubMed]
102. Van Der Pol B, Unemo M, . 2013. Chlamydial Infections, p. 55 72. In Unemo M (ed.), Laboratory diagnosis of sexually transmitted infections, including human immunodeficiency virus. World Health Organization, Geneva.
103. Gaydos CA . 2009. Can we climb out of the “pit” of poorly performing rapid diagnostic tests for chlamydia? Sex Transm Infect 85 : 158[CrossRef].[PubMed]
104. Rani R, Corbitt G, Killough R, Curless E . 2002. Is there any role for rapid tests for Chlamydia trachomatis? Int J STD AIDS 13 : 22 24[CrossRef].[PubMed]
105. Gaydos C, Hardick J . 2014. Point of care diagnostics for sexually transmitted infections: perspectives and advances. Expert Rev Anti Infect Ther 12 : 657 672[CrossRef].[PubMed]
106. Schachter J . 1999. We must be realistic in evaluating rapid diagnostic tests. Sex Transm Dis 26 : 241 242[CrossRef].[PubMed]
107. Gift TL, Pate MS, Hook EW III, Kassler WJ . 1999. The rapid test paradox: when fewer cases detected lead to more cases treated: a decision analysis of tests for Chlamydia trachomatis. Sex Transm Dis 26 : 232 240[CrossRef].[PubMed]
108. Hislop J, Quayyum Z, Flett G, Boachie C, Fraser C, Mowatt G . 2010. Systematic review of the clinical effectiveness and cost-effectiveness of rapid point-of-care tests for the detection of genital chlamydia infection in women and men. Health Technol Assess 14 : 1 97, iii–iv[CrossRef].[PubMed]
109. Pearce DM, Shenton DP, Holden J, Gaydos CA . 2011. Evaluation of a novel electrochemical detection method for Chlamydia trachomatis: application for point-of-care diagnostics. IEEE Trans Biomed Eng 58 : 755 758[CrossRef].[PubMed]
110. Melendez JH, Huppert JS, Jett-Goheen M, Hesse EA, Quinn N, Gaydos CA, Geddes CD . 2013. Blind evaluation of the microwave-accelerated metal-enhanced fluorescence ultrarapid and sensitive Chlamydia trachomatis test by use of clinical samples. J Clin Microbiol 51 : 2913 2920[CrossRef].[PubMed]
111. Verkooyen RP, Willemse D, Hiep-van Casteren SCAM, Joulandan SA, Snijder RJ, van den Bosch JMM, van Helden HPT, Peeters MF, Verbrugh HA . 1998. Evaluation of PCR, culture, and serology for diagnosis of Chlamydia pneumoniae respiratory infections. J Clin Microbiol 36 : 2301 2307.[PubMed]
112. Wang S . 2000. The microimmunofluorescence test for Chlamydia pneumoniae infection: technique and interpretation. J Infect Dis 181( Suppl 3) : S421 S425[CrossRef].[PubMed]
113. Poritz MA, Blaschke AJ, Byington CL, Meyers L, Nilsson K, Jones DE, Thatcher SA, Robbins T, Lingenfelter B, Amiott E, Herbener A, Daly J, Dobrowolski SF, Teng DHF, Ririe KM . 2011. FilmArray, an automated nested multiplex PCR system for multi-pathogen detection: development and application to respiratory tract infection. PLoS One 6 : e26047[CrossRef].[PubMed]
114. Pierce VM, Elkan M, Leet M, McGowan KL, Hodinka RL . 2011. Comparison of the Idaho Technology FilmArray System to real-time PCR for detection of respiratory pathogens in children. J Clin Microbiol 50 : 364 371.[PubMed]
115. Benitez AJ, Thurman KA, Diaz MH, Conklin L, Kendig NE, Winchell JM . 2012. Comparison of real-time PCR and a microimmunofluorescence serological assay for detection of chlamydophila pneumoniae infection in an outbreak investigation. J Clin Microbiol 50 : 151 153[CrossRef].[PubMed]
116. Gaydos CA . 2013. What is the role of newer molecular tests in the management of CAP? Infect Dis Clin North Am 27 : 49 69[CrossRef].[PubMed]
117. Hammerschlag MR, Chirgwin K, Roblin PM, Gelling M, Dumornay W, Mandel L, Smith P, Schachter J . 1992. Persistent infection with Chlamydia pneumoniae following acute respiratory illness. Clin Infect Dis 14 : 178 182[CrossRef].[PubMed]
118. Van Der Pol B, Taylor SN, Liesenfeld O, Williams JA, Hook EW III . 2013. Vaginal swabs are the optimal specimen for detection of genital Chlamydia trachomatis or Neisseria gonorrhoeae using the Cobas 4800 CT/NG test. Sex Transm Dis 40 : 247 250[CrossRef].[PubMed]
119. Blake DR, Maldeis N, Barnes MR, Hardick A, Quinn TC, Gaydos CA . 2008. Cost-effectiveness of screening strategies for Chlamydia trachomatis using cervical swabs, urine, and self-obtained vaginal swabs in a sexually transmitted disease clinic setting. Sex Transm Dis 35 : 649 655[CrossRef].[PubMed]
120. Rose SB, Lawton BA, Bromhead C, Macdonald EJ, Lund KA . 2007. Self-obtained vaginal swabs for PCR chlamydia testing: a practical alternative. Aust N Z J Obstet Gynaecol 47 : 415 418[CrossRef].[PubMed]
121. Hoebe CJ, Rademaker CW, Brouwers EE, ter Waarbeek HL, van Bergen JE . 2006. Acceptability of self-taken vaginal swabs and first-catch urine samples for the diagnosis of urogenital Chlamydia trachomatis and Neisseria gonorrhoeae with an amplified DNA assay in young women attending a public health sexually transmitted disease clinic. Sex Transm Dis 33 : 491 495[CrossRef].[PubMed]
122. Schachter J, Chernesky MA, Willis DE, Fine PM, Martin DH, Fuller D, Jordan JA, Janda W, Hook EW III . 2005. Vaginal swabs are the specimens of choice when screening for Chlamydia trachomatis and Neisseria gonorrhoeae: results from a multicenter evaluation of the APTIMA assays for both infections. Sex Transm Dis 32 : 725 728[CrossRef].[PubMed]
123. Chernesky MA, Hook EW III, Martin DH, Lane J, Johnson R, Jordan JA, Fuller D, Willis DE, Fine PM, Janda WM, Schachter. 2005. Women find it easy and prefer to collect their own vaginal swabs to diagnose Chlamydia trachomatis or Neisseria gonorrhoeae infections. Sex Transm Dis 32 : 729 733[CrossRef].[PubMed]


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Features of Various Classes of Assay for Detection of

Citation: Van Der Pol B, Gaydos C. 2016. Chlamydiae, p 545-559. In Loeffelholz M, Hodinka R, Young S, Pinsky B (ed), Clinical Virology Manual, Fifth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555819156.ch38
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Advantages and Disadvantages of Diagnostic Methodologies

Citation: Van Der Pol B, Gaydos C. 2016. Chlamydiae, p 545-559. In Loeffelholz M, Hodinka R, Young S, Pinsky B (ed), Clinical Virology Manual, Fifth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555819156.ch38

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