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Category: Microbial Genetics and Molecular Biology
Growth and Differentiation Factors, Page 1 of 2
< Previous page | Next page > /docserver/preview/fulltext/10.1128/9781555819194/9781555819187_Chap03-1.gif /docserver/preview/fulltext/10.1128/9781555819194/9781555819187_Chap03-2.gifAbstract:
Most hematologists accept that hematopoiesis is initiated by multipotential hematopoietic stem cells. These cells are regarded as being few in number but diverse in properties and as being usually noncycling in normal health. It is well documented that, when myeloid populations are damaged by irradiation or chemotherapy, hematopoietic repopulation is initiated by such stem cells.
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When stimulated in culture, BL-CFCs can each produce large colonies containing a wide variety of committed progenitor cells in different lineages. Present in many colonies are also BL-CFCs, indicating a capacity of the initiating cells to self-generate and to sustain the continuous production of maturing progeny cells. CFU-s, colony-forming unit-spleen; E, erythroid progenitors; G, granulocyte progenitors; GM, granulocyte-macrophage progenitors; M, macrophage progenitors; Eo, eosinophil progenitors; Meg, megakaryocyte progenitors; DC, dendritic cell progenitors; T, T lymphocyte progenitors; B, B lymphocyte progenitors.
When stimulated in culture, BL-CFCs can each produce large colonies containing a wide variety of committed progenitor cells in different lineages. Present in many colonies are also BL-CFCs, indicating a capacity of the initiating cells to self-generate and to sustain the continuous production of maturing progeny cells. CFU-s, colony-forming unit-spleen; E, erythroid progenitors; G, granulocyte progenitors; GM, granulocyte-macrophage progenitors; M, macrophage progenitors; Eo, eosinophil progenitors; Meg, megakaryocyte progenitors; DC, dendritic cell progenitors; T, T lymphocyte progenitors; B, B lymphocyte progenitors.
Colony formation in 7-day cultures of C57BL mouse bone marrow cells stimulated by GM-CSF. Final concentration of GM-CSF in the 1:1 cultures was 10 ng/ml. The colony numbers ± standard deviations were derived from cultures of five different bone marrow cell populations.
Colony formation in 7-day cultures of C57BL mouse bone marrow cells stimulated by GM-CSF. Final concentration of GM-CSF in the 1:1 cultures was 10 ng/ml. The colony numbers ± standard deviations were derived from cultures of five different bone marrow cell populations.
Colony formation by marrow cells from patients with acute or chronic myeloid leukemia using varying concentrations of material with colony-stimulating activity. The responsiveness of the leukemic cells to generate colonies (verified by karyotypic colony analysis) is broadly similar to that of normal human marrow cells, although some acute myeloid leukemia populations contained some cells capable of limited unstimulated proliferation.
Colony formation by marrow cells from patients with acute or chronic myeloid leukemia using varying concentrations of material with colony-stimulating activity. The responsiveness of the leukemic cells to generate colonies (verified by karyotypic colony analysis) is broadly similar to that of normal human marrow cells, although some acute myeloid leukemia populations contained some cells capable of limited unstimulated proliferation.
Colony-stimulating activity for mouse bone marrow cells of CSFs and other cytokines a
Colony-stimulating activity for mouse bone marrow cells of CSFs and other cytokines a