Chapter 13 : The Exosporium: What’s the Big “Hairy” Deal?

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In some species, including , the coat and a glycoprotein layer referred to as the spore crust are the outermost layer of the spore ( Fig. 1 ). These spore structures are discussed in more detail in Driks and Eichenberger ( ). In others, such as the family, there is an additional layer that envelops the coat, called the exosporium, which is distinct from the crust. In the case of , one of the three pathogenic species of the family, a series of fine hair-like projections, also referred to as a “hair-like” nap, extends from the exosporium basal layer ( ) ( Fig. 1 ). Other exosporium-producing species, such as , lack this “hairy” nap ( Fig. 1 ). Separating the exosporium from the rest of the spore structure is an area referred to as the interspace ( ).

Citation: Bozue J, Welkos S, Cote C. 2016. The Exosporium: What’s the Big “Hairy” Deal?, p 253-268. In Driks A, Eichenberger P (ed), The Bacterial Spore: from Molecules to Systems. ASM Press, Washington, DC. doi: 10.1128/microbiolspec.TBS-0021-2015
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Figure 1

Electron micrographs of , and spores. The spore coat (white bar) or exosporium (black bar) as present is labeled for the various species. The arrow indicates the presence of a crust on the spore. The micrograph is courtesy of Adam Driks, Stritch School of Medicine, Loyola University Chicago, Maywood, IL.

Citation: Bozue J, Welkos S, Cote C. 2016. The Exosporium: What’s the Big “Hairy” Deal?, p 253-268. In Driks A, Eichenberger P (ed), The Bacterial Spore: from Molecules to Systems. ASM Press, Washington, DC. doi: 10.1128/microbiolspec.TBS-0021-2015
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Figure 2

Association of spores to bronchial epithelial cells. Micrographs of spores associated with the bronchial epithelial cells. Samples were stained with spore stain (malachite green) and counterstained with a Wright-Giemsa stain. Ames spores (indicated by white arrows) with the cells. mutant spores with the cells. Reprinted from reference with permission.

Citation: Bozue J, Welkos S, Cote C. 2016. The Exosporium: What’s the Big “Hairy” Deal?, p 253-268. In Driks A, Eichenberger P (ed), The Bacterial Spore: from Molecules to Systems. ASM Press, Washington, DC. doi: 10.1128/microbiolspec.TBS-0021-2015
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Figure 3

Scanning and transmission electron micrographs of the interaction between spores and bronchial epithelial cells. Adherence of Ames spores to cells 2 h postinfection. Bar corresponds to 5 µm. Adherence of mutant spores to cells 2 h postinfection. Bar corresponds to 10 µm. mutant spores incubated with cells. Bar corresponds to 5 µm. Close-up view from boxed area in panel C. Bar corresponds to 0.5 µm. Reprinted from reference with permission.

Citation: Bozue J, Welkos S, Cote C. 2016. The Exosporium: What’s the Big “Hairy” Deal?, p 253-268. In Driks A, Eichenberger P (ed), The Bacterial Spore: from Molecules to Systems. ASM Press, Washington, DC. doi: 10.1128/microbiolspec.TBS-0021-2015
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