Chapter 32 : Donna M. Wolk: It’s Never Too Late To Bloom

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Donna M. Wolk pursued her higher degree later in life than many of her counterparts, yet in a short time she has achieved much success in clinical microbiology, contributing to the widespread use of molecular diagnostics in the clinical microbiology laboratory. Her dedication to improving health care for patients by implementing rapid, infectious disease diagnostics has allowed her to cross paths with many other scientists, both young and old. Those who do cross paths with Donna will likely reap the benefits of her jovial, brilliant, and giving personality, which indeed leaves an everlasting impression, both professionally and personally.

Citation: Whitfield N. 2018. Donna M. Wolk: It’s Never Too Late To Bloom, p 295-304. In Whitaker R, Barton H (ed), Women in Microbiology. ASM Press, Washington, DC. doi: 10.1128/9781555819545.ch32
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Image of FIGURE 1

Donna Wolk observes as a laboratory scientist, Candi Grado, uses a microscope to read a fluorescent peptide nucleic acid FISH test. Reprinted with permission from UANews. Photo credit: Beatriz Verdugo/UANews.

Citation: Whitfield N. 2018. Donna M. Wolk: It’s Never Too Late To Bloom, p 295-304. In Whitaker R, Barton H (ed), Women in Microbiology. ASM Press, Washington, DC. doi: 10.1128/9781555819545.ch32
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1. Marshall MM,, Naumovitz D,, Ortega Y,, Sterling CR . 1997. Waterborne protozoan pathogens. Clin Microbiol Rev 10 : 67 85.
2. Wolk DM,, Johnson CH,, Rice EW,, Marshall MM,, Grahn KF,, Plummer CB,, Sterling CR . 2000. A spore counting method and cell culture model for chlorine disinfection studies of Encephalitozoon syn. Septata intestinalis. Appl Environ Microbiol 66 : 1266 1273.
3. Wolk DM,, Jones MF,, Rosenblatt JE . 2001. Laboratory diagnosis of viral hepatitis. Infect Dis Clin North Am 15 : 1109 1126.
4. Baghai M,, Osmon DR,, Wolk DM,, Wold LE,, Haidukewych GJ,, Matteson EL . 2001. Fatal sepsis in a patient with rheumatoid arthritis treated with etanercept. Mayo Clin Proc 76 : 653 656.
5. Wolk DM,, Picton E,, Johnson D,, Davis T,, Pancholi P,, Ginocchio CC,, Finegold S,, Welch DF,, de Boer M,, Fuller D,, Solomon MC,, Rogers B,, Mehta MS,, Peterson LR . 2009. Multicenter evaluation of the Cepheid Xpert methicillin-resistant Staphylococcus aureus (MRSA) test as a rapid screening method for detection of MRSA in nares. J Clin Microbiol 47 : 758 764.
6. Wolk DM,, Struelens MJ,, Pancholi P,, Davis T,, Della-Latta P,, Fuller D,, Picton E,, Dickenson R,, Denis O,, Johnson D,, Chapin K . 2009. Rapid detection of Staphylococcus aureus and methicillin-resistant S. aureus (MRSA) in wound specimens and blood cultures: multicenter preclinical evaluation of the Cepheid Xpert MRSA/SA skin and soft tissue and blood culture assays. J Clin Microbiol 47 : 823 826.
7. Wolk DM,, Marx JL,, Dominguez L,, Driscoll D,, Schifman RB . 2009. Comparison of MRSASelect agar, CHROMagar methicillin-resistant Staphylococcus aureus (MRSA) medium, and Xpert MRSA PCR for detection of MRSA in nares: diagnostic accuracy for surveillance samples with various bacterial densities. J Clin Microbiol 47 : 3933 3936.
8. Kaleta EJ,, Clark AE,, Johnson DR,, Gamage DC,, Wysocki VH,, Cherkaoui A,, Schrenzel J,, Wolk DM . 2011. Use of PCR coupled with electrospray ionization mass spectrometry for rapid identification of bacterial and yeast bloodstream pathogens from blood culture bottles. J Clin Microbiol 49 : 345 353.
9. Marner ES,, Wolk DM,, Carr J,, Hewitt C,, Dominguez LL,, Kovacs T,, Johnson DR,, Hayden RT . 2011. Diagnostic accuracy of the Cepheid GeneXpert vanA/vanB assay ver. 1.0 to detect the vanA and vanB vancomycin resistance genes in Enterococcus from perianal specimens. Diagn Microbiol Infect Dis 69 : 382 389.
10. Kaleta EJ,, Clark AE,, Cherkaoui A,, Wysocki VH,, Ingram EL,, Schrenzel J,, Wolk DM . 2011. Comparative analysis of PCR-electrospray ionization/mass spectrometry (MS) and MALDI-TOF/MS for the identification of bacteria and yeast from positive blood culture bottles. Clin Chem 57 : 1057 1067.
11. Schieffer KM,, Tan KE,, Stamper PD,, Somogyi A,, Andrea SB,, Wakefield T,, Romagnoli M,, Chapin KC,, Wolk DM,, Carroll KC . 2014. Multicenter evaluation of the Sepsityper™ extraction kit and MALDI-TOF MS for direct identification of positive blood culture isolates using the BD BACTEC™ FX and VersaTREK ® diagnostic blood culture systems. J Appl Microbiol 116 : 934 941.
12. Stoneking LR,, Patanwala AE,, Winkler JP,, Fiorello AB,, Lee ES,, Olson DP,, Wolk DM . 2013. Would earlier microbe identification alter antibiotic therapy in bacteremic emergency department patients? J Emerg Med 44 : 1 8.
13. Wolk DM,, Kaleta EJ,, Wysocki VH . 2012. PCR-electrospray ionization mass spectrometry. J Mol Diagn 14 : 295 304.
14. Buehler SS,, Madison B,, Snyder SR,, Derzon JH,, Cornish NE,, Saubolle MA,, Weissfeld AS,, Weinstein MP,, Liebow EB,, Wolk DM . 2016. Effectiveness of practices to increase timeliness of providing targeted therapy for inpatients with bloodstream infections: a laboratory medicine best practices systematic review and meta-analysis. Clin Microbiol Rev 29 : 59 103.
15. Stolte D . 2011. Making germs glow: new test helps save lives and cuts costs. UANews, Tucson, AZ.
16. National Institutes of Health . 2010. NOT-OD-10-047: Ruth L. Kirschstein National Research Service Award (NRSA) stipends, tuition/fees and other budgetary levels effective for fiscal year 2010. National Institutes of Health, Bethesda, MD.

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