Chapter 14 : Cell Lines: Applications and Biosafety

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Animal cells have been used in biotechnology since the early 1950s. The Salk polio vaccine, licensed in 1954, was the first product produced on animal cells as a substrate, and for many years the only products produced using animal cells were viral vaccines. Primary animal cells were used for many years for vaccine production and are still used in certain cases. These vaccines have generally proven to be acceptable and safe, but there are notable exceptions that have directed manufacturers and regulatory bodies to be very cautious in their assessment of new cell substrates. The earliest cell lines used to manufacture biological products were human diploid fibroblast finite cell lines. WI-38 and MRC-5, two of the best-known examples, have been used in the manufacture of a number of licensed products (Table 1). The early use of continuous cell lines (CCLs) for the manufacture of biological products is represented by the manufacture of foot-and-mouth disease vaccine in the Syrian hamster cell line BHK, the production of interferon from the B-lymphoblastoid cell line Namalwa, and the introduction of monoclonal antibodies from hybridoma cells in the 1990s (for a general reference, see reference 1).

Citation: Stacey G, Hawkins J. 2017. Cell Lines: Applications and Biosafety, p 299-325. In Wooley D, Byers K (ed), Biological Safety: Principles and Practices, Fifth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555819637.ch14
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Generic image for table
Table 1.

Citation: Stacey G, Hawkins J. 2017. Cell Lines: Applications and Biosafety, p 299-325. In Wooley D, Byers K (ed), Biological Safety: Principles and Practices, Fifth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555819637.ch14
Generic image for table
Table 2.

Citation: Stacey G, Hawkins J. 2017. Cell Lines: Applications and Biosafety, p 299-325. In Wooley D, Byers K (ed), Biological Safety: Principles and Practices, Fifth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555819637.ch14
Generic image for table
Table 3.

Citation: Stacey G, Hawkins J. 2017. Cell Lines: Applications and Biosafety, p 299-325. In Wooley D, Byers K (ed), Biological Safety: Principles and Practices, Fifth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555819637.ch14
Generic image for table
Table 4.

Citation: Stacey G, Hawkins J. 2017. Cell Lines: Applications and Biosafety, p 299-325. In Wooley D, Byers K (ed), Biological Safety: Principles and Practices, Fifth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555819637.ch14

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