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Part III : Basics of Molecular Methods for Fungal Identification

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Citation: Das S. 2018. Basics of Molecular Methods for Fungal Identification, p 333-358. In Walsh T, Hayden R, Larone D, Larone’s Medically Important Fungi: A Guide to Identification, Sixth Edition. ASM Press, Washington, DC.
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Figures

Image of FIGURE 1
FIGURE 1

Ribosomal DNA (rDNA) gene complex.

Citation: Das S. 2018. Basics of Molecular Methods for Fungal Identification, p 333-358. In Walsh T, Hayden R, Larone D, Larone’s Medically Important Fungi: A Guide to Identification, Sixth Edition. ASM Press, Washington, DC.
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Image of FIGURE 2
FIGURE 2

Molecular beacon hairpin-loop structure. F, fluorophore; Q, quencher.

Citation: Das S. 2018. Basics of Molecular Methods for Fungal Identification, p 333-358. In Walsh T, Hayden R, Larone D, Larone’s Medically Important Fungi: A Guide to Identification, Sixth Edition. ASM Press, Washington, DC.
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Download as Powerpoint
Image of FIGURE 3
FIGURE 3

Hybridization of molecular beacon probe to target DNA with resultant fluorescence. F, fluorophore; Q, quencher.

Citation: Das S. 2018. Basics of Molecular Methods for Fungal Identification, p 333-358. In Walsh T, Hayden R, Larone D, Larone’s Medically Important Fungi: A Guide to Identification, Sixth Edition. ASM Press, Washington, DC.
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Download as Powerpoint
Image of FIGURE 4
FIGURE 4

Sample phylogenetic tree of select spp. Strain designations are shown at the end of each branch. (Condensed and adapted from Zeng et al., 2007.) To comprehend the evolutionary branching of the isolates, the tree should be viewed from the left to the right. Each bifurcation (branching) of the tree indicates likely evolutionary divergence. The number (known as the bootstrap value) at the point of bifurcation is a percentage based on numerous repeat sequence analyses and indicates the mathematical strength of the grouping; bootstrap values of .90 are shown at the branches. As the tree branches to the right, more closely related isolates ultimately cluster on the same or neighboring branches and are designated in the phylogram as one species. Nucleotide changes that have taken place, expressed as distance, are shown by the scale bar.

Citation: Das S. 2018. Basics of Molecular Methods for Fungal Identification, p 333-358. In Walsh T, Hayden R, Larone D, Larone’s Medically Important Fungi: A Guide to Identification, Sixth Edition. ASM Press, Washington, DC.
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