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Chapter 11 : Gram-Positive Type IV Pili and Competence

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Gram-Positive Type IV Pili and Competence, Page 1 of 2

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Abstract:

In recent years, numerous complete bacterial genome sequences became available and led to the identification of surprisingly diverse type IV pili (T4P) across a broad range of Gram-positive bacteria. The genes encoding T4P components cluster together in distinct loci, and three subsets of T4P loci present in Gram-positive bacteria have been described: (in) loci, (petence) loci, and (ight herence) loci ( ). Interestingly, they are not mutually exclusive. In fact, many Gram-positive bacteria harbor a combination of , , and loci, suggesting diverse functional roles for T4P in Gram-positive bacteria ( ). loci are commonly found in spp., and clostridial T4P are best studied for and ( ). loci are widespread in Firmicutes, among Bacillales and Lactobacillales. loci are present in archaea and Gram-negative and Gram-positive bacteria. Proteins of the system assemble adhesive fimbrial low-molecular-weight protein (Flp) pili that are largely unexplored in Gram-positive bacteria ( ). and loci are not extensively discussed here. Instead we focus on the operon present in the human respiratory pathogen and containing the genes involved in the formation of the pneumococcal type IV pilus, also referred to as competence pilus or transformation pilus.

Citation: Muschiol S, Aschtgen M, Nannapaneni P, Henriques-Normark B. 2019. Gram-Positive Type IV Pili and Competence, p 129-135. In Sandkvist M, Cascales E, Christie P (ed), Protein Secretion in Bacteria. ASM Press, Washington, DC. doi: 10.1128/microbiolspec.PSIB-0011-2018
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Figures

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Figure 1

Type IV competence pilus produced by R6. The pilus was visualized by transmission electron microscopy after negative staining with uranyl acetate. Scale bar, 100 nm.

Citation: Muschiol S, Aschtgen M, Nannapaneni P, Henriques-Normark B. 2019. Gram-Positive Type IV Pili and Competence, p 129-135. In Sandkvist M, Cascales E, Christie P (ed), Protein Secretion in Bacteria. ASM Press, Washington, DC. doi: 10.1128/microbiolspec.PSIB-0011-2018
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Image of Figure 2
Figure 2

Schematic representation of the DNA uptake machinery in competent . Upon competence induction, the pneumococcal competence pilus composed of ComGC is made and captures extracellular, transforming DNA ( ). Captured DNA is passed to the DNA receptor ComEA and the transmembrane channel protein ComEC. This step is possibly mediated by an unknown retraction ATPase and subsequent pilus retraction or yet another undescribed mechanism. Double-stranded DNA (dsDNA) is cleaved by the endonuclease EndA and single-stranded DNA (ssDNA) enters the cytoplasm through the ComEC pore driven by the DNA-dependent ATPase ComFA ( ). ComFA forms a complex with ComFC and DprA ( ) and together with SsbB and RecA help in stabilization and processing of internalized ssDNA to facilitate genetic exchange ( ).

Citation: Muschiol S, Aschtgen M, Nannapaneni P, Henriques-Normark B. 2019. Gram-Positive Type IV Pili and Competence, p 129-135. In Sandkvist M, Cascales E, Christie P (ed), Protein Secretion in Bacteria. ASM Press, Washington, DC. doi: 10.1128/microbiolspec.PSIB-0011-2018
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