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Chapter 7 : Protein Secretion in Spirochetes

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Abstract:

Spirochetes form a distinct bacterial phylum of slender, diderm (dual-membrane) bacteria that exhibit either a coiled “corkscrew” or flat-wave “serpentine” morphology. These distinct phenotypes are at least partly due to various numbers of periplasmic flagella that are inserted subterminally at both poles of the bacteria, wrapping around the protoplasmic cylinder and often overlapping in the middle of the cell. Coordinated rotation of the flagellar bands or bundles, sometimes referred to as axial filaments, leads to rotation of the cell cylinder and cellular motility that is particularly prominent in viscous environments.

Citation: Zückert W. 2019. Protein Secretion in Spirochetes, p 77-89. In Sandkvist M, Cascales E, Christie P (ed), Protein Secretion in Bacteria. ASM Press, Washington, DC. doi: 10.1128/microbiolspec.PSIB-0026-2019
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Figures

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Figure 1

Envelope structures of model spirochetes , , and . Spirochetes have a common diderm envelope structure with an inner membrane (IM) and outer membrane (OM) and a periplasmic space in between that contains a thin peptidoglycan cell wall and periplasmic flagella. A major difference between the three genera is seen in the OM: displays a limited set of integral OM proteins (OMPs), among them the unusual porin P13 with a predicted α-helical TM topology, but a large variable set of surface lipoproteins. The OM most closely resembles a Gram-negative OM, with lipopolysaccharide (LPS) being the major component of the surface leaflet, complemented by a large number of OMPs and a limited set of surface lipoproteins. expresses only rare OMPs with limited surface exposure. Some of the model proteins under study are labeled in italics. See text for details.

Citation: Zückert W. 2019. Protein Secretion in Spirochetes, p 77-89. In Sandkvist M, Cascales E, Christie P (ed), Protein Secretion in Bacteria. ASM Press, Washington, DC. doi: 10.1128/microbiolspec.PSIB-0026-2019
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Image of Figure 2
Figure 2

Generalized model of spirochetal protein secretion. The left half shows type I to III secretion systems (T1SS, T2SS, and T3SS) as well as the pathways involved in the secretion of nonlipidated membrane and periplasmic proteins. The right half shows the pathways for lipoprotein (LP) modification, sorting, and secretions. Pathway components or mechanisms that appear unique to a particular spirochetal genus are labeled by stippled red circles with the genus initial: , ; , ; and , . For example, a potential T2SS has been identified only for . The pathways in blue delineate the current alternative periplasmic and OM mechanisms that may be involved in the secretion of α-helical integral OMPs and surface lipoproteins in ; of note, could also take advantage of its T2SS to secrete surface lipoproteins ( ). Release of outer membrane vesicles (OMVs) has been observed and studied in . See text for details.

Citation: Zückert W. 2019. Protein Secretion in Spirochetes, p 77-89. In Sandkvist M, Cascales E, Christie P (ed), Protein Secretion in Bacteria. ASM Press, Washington, DC. doi: 10.1128/microbiolspec.PSIB-0026-2019
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