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Cell Wars: a Laboratory Model for Macrophage Phagocytosis of Gram-Negative Bacteria
MyBook is a cheap paperback edition of the original book and will be sold at uniform, low price.
Students culture mouse macrophage cells to confluence in a six-well plate and then unleash these cells on cultured Escherichia coli bacteria.
Three aspects of phagocytosis are examined:
- Visualizing E. coli within the macrophage.
- Determining how many bacteria are ingested by the macrophage cells.
- Determining the number of bacteria still viable after a 1-hour exposure to the macrophages’ lethal oxidative abilities.
A simple quantitative methodology for evaluating host-microbe interaction is introduced. Assessment is a review of the student’s laboratory notebook, questions in the handout, experimental results, and a brief laboratory report that integrates data analysis with discussion.