Screening for the Antibacterial Activity of Medicinal Plants

  • Authors: Delia de Castro-Ontengco1, Teresita Capal2
    Affiliations: 1: Graduate School, University of Sto. Tomas, España, Manila, Biological Sciences Dept., Medical Affairs Division, United Laboratories; 2: Natural Products Testing Laboratory, Industrial Technology Dev’t Inst, Dept of Science and Technology
  • Citation: Delia de Castro-Ontengco, Teresita Capal. 2004. Screening for the antibacterial activity of medicinal plants.
  • Publication Date : April 2004
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Plants with suspected medicinal value will be identified, collected, and dried. Organic plant compounds will be extracted with ethanol. The plant extract will be dispensed to blank disks and dried to evaporate the solvent. The antibacterial activity of the plant extracts will be determined using the Kirby-Bauer method. A standardized bacterial suspension will be swabbed uniformly onto the surface of the Mueller-Hinton agar. The plant disks will be placed at equidistant points on the agar surface, and plates will be incubated at 35oC for 24 hours. Zones of inhibition, indicating antibacterial activity, will be measured in millimeters.

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