Do-It-Yourself Immunoglobulin Gene Rearrangement

  • Author: Wendy Gorman1
    Affiliations: 1: Biology, Northland College
  • Citation: Wendy Gorman. 2002. Do-it-yourself immunoglobulin gene rearrangement.
  • Publication Date : September 2002
MyBook is a cheap paperback edition of the original book and will be sold at uniform, low price.
You must be logged in to use this functionality

This exercise provides hands-on experience in visualizing and understanding the difficult concept of immunoglobulin gene rearrangment and isotype switching. Students loop, ligate (tape), and cut strips of paper DNA to simulate the looping and joining of heavy chain gene segments. Students begin with a strip of germ line DNA, rearrange the DNA, form a primary RNA transcript, process it into mRNA, translate a nascent polypeptide, and produce the final heavy chain. At the completion of this activity students will understand immunoglobulin gene rearrangements and isotype switching enough to start with nascent DNA and create any isotype of immunoglobulin.

Executive Summary

No executive summary material available for this content.

Supplemental Material

No supplementary material available for this content.

This is a required field
Please enter a valid email address
Please check the format of the address you have entered.
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error