Kirby-Bauer Disk Diffusion Susceptibility Test
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Kirby-Bauer disk diffusion susceptibility test
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Kirby-Bauer disk diffusion susceptibility test
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Kirby-Bauer disk diffusion susceptibility test
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Kirby-Bauer disk diffusion susceptibility test
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Kirby-Bauer disk diffusion susceptibility test
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Kirby-Bauer disk diffusion susceptibility test
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Kirby-Bauer disk diffusion susceptibility test
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Kirby-Bauer disk diffusion susceptibility test
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Kirby-Bauer disk diffusion susceptibility test
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Kirby-Bauer disk diffusion susceptibility test
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Kirby-Bauer disk diffusion susceptibility test
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Kirby-Bauer disk diffusion susceptibility test
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Kirby-Bauer disk diffusion susceptibility test
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Kirby-Bauer disk diffusion susceptibility test
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Kirby-Bauer disk diffusion susceptibility test
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McFarland standards
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Inoculation of the test plate
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Inoculation of the Mueller-Hinton agar plate
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Diagram illustrating the pattern the swab should follow as it is drawn across the plate
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Inoculation of the Mueller-Hinton agar plate
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Set the dispenser over the plate
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Place the palm of your hand on the top of the handle
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Press down firmly and completely to dispense the disks
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Place the Mueller-Hinton agar plate over the disk template
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Remove one disk from the cartridge using forceps that have been sterilized
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Lift the lid of the plate and place the disk over one of the positioning marks
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Press the disk with the forceps to ensure complete contact with the agar surface. Replace the lid of the plate between disks to minimize exposure to airborne contaminants.
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Manual disk placement template for 8 disks on a 100-mm plate
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Measuring zones of inhibition: gray shading represents a confluent lawn of bacterial growth
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Using a ruler or caliper measure each zone with the unaided eye while viewing the back of the petri dish. Hold the plate a few inches above a black, non-reflecting surface illuminated with reflected light.
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The size of the zone for this organism-antimicrobial combination is 26 mm
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Alternate method for measuring zones
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Staphylococcus saprophyticus resistant to novobiocin
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Staphylococcus epidermidis is sensitive with a zone of inhibition
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Staphylococcus aureus is sensitive with a zone of inhibition
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Bacitracin test