Herpes Simplex Virus Type 1 Infection of Primary Rabbit Kidney Cells

  • Author: Howell Rogers
  • Citation: Howell Rogers. 2009. Herpes simplex virus type 1 infection of primary rabbit kidney cells.
  • Publication Date : December 2009
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Photomicrographs of herpes simplex virus (HSV) type 1-infected primary rabbit kidney cells collected from human vesicle fluid. Inoculation of primary rabbit kidney cell monolayers is a sensitive and efficient way to detect HSV in human clinical specimens. Following infection, monolayers may be examined periodically until cytopathic effects (CPE), either rounding and/or detachment of cells or presence of multinucleate giant cells, are evident. The photomicrographs above show primary rabbit kidney cells at 48 h post infection. Following methanol fixation, cells were visualized by the Giemsa staining technique. In Figure 1 (uninfected cells, 100x) a typical monolayer is evident. At 400x magnification, uninfected cells exhibit distinct nucleoli (Fig. 2) within nuclei. By contrast, Figure 3 (HSV-1 infected cells, 100x) shows viral cytopathic effect in which multinucleated giant cells have formed due to fusion of infected cells. The nucleoli are much smaller and less evident and the emargination of chromatin is discernable (Fig. 4, HSV-1 infected cells, 400x) as a result of infection. Since respiratory syncytial virus, measles virus, and herpes zoster virus do not grow in rabbit kidney cells, presence of this type CPE is presumptive evidence of HSV. The virus was confirmed by direct fluorescent monoclonal antibody typing and shown to be HSV type 1 (not shown).

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