Primary Chicken Embryo Liver Cells in Culture

  • Author: Patrick J. Cummings
  • Citation: Patrick J. Cummings. 2009. Primary chicken embryo liver cells in culture.
  • Publication Date : December 2009
MyBook is a cheap paperback edition of the original book and will be sold at uniform, low price.
You must be logged in to use this functionality

Primary cells are harvested directly from an organism and can be grown for several weeks in vitro in specialized cell culture medium before the cells undergo senescence. Primary cells are especially sensitive to chemicals, toxins, and viruses, and are often used for various research and industrial applications. 

This phase-contrast image shows primary chick embryo liver cells grown for 2 weeks in vitro. Note the liver cell nuclei (blue) and numerous cytoplasmic vacuoles containing glycogen.

Livers were harvested from 10-day-old embryonated chicken eggs. The liver was dissected from the embryo using aseptic technique, followed by tissue mincing, trypsinizing, and seeding into T25 tissue culture flasks. The cells were grown at 37oC under 5% CO2in hepatocyte basal medium (Cambrex Bio Science) for 2 weeks.

Related Resources

This is a required field
Please enter a valid email address
Please check the format of the address you have entered.
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error