Eosin-Methylene Blue Agar Plates Protocol
- Authors: Archana Lal 1, Naowarat Cheeptham 2
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VIEW AFFILIATIONS HIDE AFFILIATIONSAffiliations: 1: Independence Community College, Independence, KS, 67301; 2: Thompson Rivers University, Kamloops, BC V2C 5N3
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Citation: Archana Lal, Naowarat Cheeptham. 2007. Eosin-methylene blue agar plates protocol.
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Publication Date : September 2007
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Category: Protocol
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Eosin-Methylene Blue Agar Plates Protocol
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Citation:
Archana Lal, Naowarat Cheeptham. 2007. Eosin-methylene blue agar plates protocol.
/content/education/protocol/2869.f-1
Figure 1
FIG. 1. Nutrient agar inoculated with Escherichia coli (a gram-negative bacterium) demonstrating growth with whitish colonies.
education/protocol/images/atlas_emb/escherichia-coli_embprotfig1.jpg
education/protocol/images/atlas_emb/escherichia-coli_embprotfig1.jpg
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Figure 1
FIG. 1. Nutrient agar inoculated with Escherichia coli (a gram-negative bacterium) demonstrating growth with whitish colonies.
Source:
September 2007
/content/education/protocol/2869.f-2
Figure 2
FIG. 2. Eosin-methylene blue (
EMB) agar plate inoculated with Escherichia coli (a gram-negative coliform bacterium) showing good growth of dark blue-black colonies with metallic green sheen indicating vigorous fermentation of lactose and acid production which precipitates the green metallic pigment. (Naowarat Cheeptham, Thompson Rivers University, Kamloops, BC, Canada)
education/protocol/images/2073_e.coli_emb_fig1.jpg
education/protocol/images/atlas_emb/e.coli_emb_fig1.jpg
Click to view
Figure 2
FIG. 2. Eosin-methylene blue ( EMB) agar plate inoculated with Escherichia coli (a gram-negative coliform bacterium) showing good growth of dark blue-black colonies with metallic green sheen indicating vigorous fermentation of lactose and acid production which precipitates the green metallic pigment. (Naowarat Cheeptham, Thompson Rivers University, Kamloops, BC, Canada)
Source:
September 2007
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Figure 3
FIG. 3. Nutrient agar inoculated with Pseudomonas aeruginosa (a gram-negative bacterium) demonstrating growth with whitish colonies.
education/protocol/images/atlas_emb/pseudomonas-aeruginosa_embprotfig3.jpg
education/protocol/images/atlas_emb/pseudomonas-aeruginosa_embprotfig3.jpg
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Figure 3
FIG. 3. Nutrient agar inoculated with Pseudomonas aeruginosa (a gram-negative bacterium) demonstrating growth with whitish colonies.
Source:
September 2007
/content/education/protocol/2869.f-2075
Figure 4
FIG. 4. EMB agar plate inoculated with Pseudomonas aeruginosa (a gram-negative noncoliform bacterium) showing good growth but no fermentation of sugars or acid production. (Naowarat Cheeptham and Carolynne Fardy, Thompson Rivers University, Kamloops, BC, Canada)
education/protocol/images/2075_pseudomonas-aeruginosa_emb_fig4.jpg
education/protocol/images/atlas_emb/pseudomonas-aeruginosa_emb_fig4.jpg
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Figure 4
FIG. 4. EMB agar plate inoculated with Pseudomonas aeruginosa (a gram-negative noncoliform bacterium) showing good growth but no fermentation of sugars or acid production. (Naowarat Cheeptham and Carolynne Fardy, Thompson Rivers University, Kamloops, BC, Canada)
Source:
September 2007
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Figure 5
FIG. 5. Nutrient agar inoculated with Staphylococcus aureus (a gram-positive bacterium) demonstrating growth with whitish colonies.
education/protocol/images/atlas_emb/staphylococcus-aureus_embprotfig5.jpg
education/protocol/images/atlas_emb/staphylococcus-aureus_embprotfig5.jpg
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Figure 5
FIG. 5. Nutrient agar inoculated with Staphylococcus aureus (a gram-positive bacterium) demonstrating growth with whitish colonies.
Source:
September 2007
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Figure 6
education/protocol/images/atlas_emb/staphylococcus-aureus_embprot_fig6.jpg
education/protocol/images/atlas_emb/staphylococcus-aureus_embprot_fig6.jpg
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Figure 6
Source:
September 2007