Cytopathic Effects of Viruses Protocols

Figure 1

FIG. 1. Bovine fetal spleen cells uninfected

Figure 2

FIG. 1. Unstained bovine fetal spleen cells 1 day postinfection with a high multiplicity of infection (MOI) of bovine enterovirus, a Picornavirus, showing pyknotic shrinking cells. Cells were observed using the 10X objective. Note: this shows viral CPE within 24 hours, within 48 hours all of the cells will be dead and detached. (Lori Phalen and Carol Blair, Colorado State University, Fort Collins, CO)

Figure 3

FIG. 2. Unstained bovine fetal spleen cells 2 days postinfection with a high MOI of vesicular stomatitis virus, a Rhabdovirus, showing subtotal cell destruction; the cells appear pyknotic. Cells were observed using the 10X objective. (Lori Phalen and Carol Blair, Colorado State University, Fort Collins, CO)

Figure 4

FIG. 3. Unstained bovine fetal spleen cells 2 days postinfection with a high MOI of bovine herpesvirus 1, a Herpesvirus. Cells were observed using the 10X objective. Black arrows point to cell rounding in a focal pattern and blue arrows point to cytoplasmic stranding. (Lori Phalen and Carol Blair, Colorado State University, Fort Collins, CO)

Figure 5

FIG. 4. Unstained bovine fetal spleen cells 2 days postinfection with a high MOI of Orf virus, a Parapoxvirus. Blue arrows point to cell rounding in a focal pattern. Cells were observed using the 10X objective. (Lori Phalen and Carol Blair, Colorado State University, Fort Collins, CO)

Figure 6

FIG. 6. Unstained bovine fetal spleen cells 3 days postinfection with Orf virus, a Parapoxvirus, showing focal cell rounding at a low MOI. Cells were observed using a 4X objective. Note: you can see the CPE is focal at the low MOI, but it appears generalized at the high MOI. (Erica Suchman, Colorado State University, Fort Collins, CO)

Figure 7

FIG. 7. Unstained bovine fetal spleen cells 3 days postinfection with Orf virus, a Parapoxvirus, showing focal cell rounding at a high MOI. Cells were observed using a 4X objective. Note: you can see the CPE is focal at the low MOI, but it appears generalized at the high MOI. (Erica Suchman, Colorado State University, Fort Collins, CO)

Figure 8

FIG. 8. Unstained bovine fetal spleen cells 3 days postinfection with Orf virus, a Parapoxvirus, showing focal cell rounding at a low MOI. Cells were observed using a 10X objective. (Erica Suchman, Colorado State University, Fort Collins, CO)

Figure 9

FIG. 9. Unstained bovine fetal spleen cells 3 days postinfection with Orf virus, a Parapoxvirus, showing focal cell rounding at a high MOI. Cells were observed using a 10X objective. Note: the focal nature of the infection is easier to see under lower power magnification. (Erica Suchman, Colorado State University, Fort Collins, CO)

Figure 10

FIG. 10. Unstained bovine fetal spleen cells 4 days postinfection with a high MOI of bovine adenovirus, an Adenovirus, showing cell rounding and small amounts of clumping. Cells were observed using the 10X objective. (Erica Suchman, Colorado State University, Fort Collins, CO)

Figure 11

Figure 12

FIG. 12. Giemsa-stained bovine fetal spleen cells 4 days postinfection with the bovine respiratory syncytial virus, a Paramyxovirus, showing syncytia (arrows) and faint basophilic cytoplasmic inclusion bodies (dashed arrows). Cells were observed with the 20X objective (200X total magnification). For a full description, see Giemsa-Stained Bovine Respiratory Syncytial Virus (Paramyxoviridae)-Infected Bovine Fetal Spleen Cells Showing Cytopathic Effects and Inclusions.

Figure 13

Unstained bovine fetal spleen cells infected with the parainfluenza virus type 3, a Paramyxovirus,showing a syncytium (arrows). Cells were observed using the 10X objective. (Lori Phalen and Carol Blair, Colorado State University, Fort Collins, CO)



Black and white unstained cells

Figure 14

FIG. 14. Giemsa-stained bovine fetal spleen cells 3 days postinfection with bovine herpes virus 2, a Herpesvirus, showing syncytia and nuclear inclusion bodies (arrow). Cells were observed with the 40X objective (400X total magnification). For a full description, see Giemsa-Stained Bovine Herpes Simplex Virus Type 2 (Herpesviridae)-Infected Bovine Fetal Spleen Cells Showing Cytopathic Effects and Inclusions.

Figure 15

FIG. 15. Giemsa-stained bovine fetal spleen cells 1 day postinfection with bovine herpes virus 1, a Herpesvirus, showing cytoplasmic stranding (arrow) and nuclear inclusion bodies (dashed arrow). Cells were observed with the 20X objective (200X total magnification). For a full description, see Giemsa-Stained Bovine Herpes Simplex Virus Type 1 (Herpesviridae)-Infected Bovine Fetal Spleen Cells Showing Cytopathic Effects.

Figure 16

FIG. 16. Giemsa-stained bovine fetal spleen cells 4 days postinfection with bovine adenovirus, an Adenovirus, showing rough-edged nuclear inclusion bodies (arrow). Cells were observed with the 20X objective (200X total magnification). For a full description, see Giemsa-Stained Bovine Adenovirus (Adenoviridae)-Infected Bovine Fetal Spleen Cells Showing Inclusions.

Figure 17

FIG. 17. Giemsa-stained bovine fetal spleen cells 1 day postinfection with Orf virus, a Poxvirus, showing pink eosynophilic cytoplasmic inclusion bodies (arrows) and cell swelling near the top of the field. Cells were observed using the 20X objective (200X total magnification). For a full description, see Giemsa-Stained Orf Virus (Poxviridae)-Infected Bovine Fetal Spleen Cells Showing Cytopathic Effects and Inclusions.

Figure 18

FIG. 18. A Giemsa-stained feline nasal turbinate primary cell culture infected with feline herpes virus-1, a Herpesvirus, showing chromatin margination (dark ring around the edge of the nucleus). Cells were observed with the 20X objective (200X total magnification). For a full description, see Feline Herpes Virus Diagnostics.