Capsule Stain Protocols
- Authors: Roxana B. Hughes 1, Ann C. Smith 2
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VIEW AFFILIATIONS HIDE AFFILIATIONSAffiliations: 1: Department of Biological Sciences, University of North Texas, Denton, TX, 76203; 2: Department of Cell Biology and Molecular Genetics, University of Maryland, College Park, MD, 20742
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Citation: Roxana B. Hughes, Ann C. Smith. 2007. Capsule stain protocols.
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Publication Date : September 2007
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Category: Protocol

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Figures
FIG. 1. Encapsulated Bacillus sp. grown on skim milk medium and stained using Maneval's method. (Ann C. Smith, University of Maryland, College Park, MD)

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Figure 1
FIG. 1. Encapsulated Bacillus sp. grown on skim milk medium and stained using Maneval's method. (Ann C. Smith, University of Maryland, College Park, MD)
FIG. 2. Encapsulated Streptococcus lactis stained with Anthony's capsule stain. (Gary E. Kaiser, The Community College of Baltimore County, Catonsville campus, Baltimore, MD)

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Figure 2
FIG. 2. Encapsulated Streptococcus lactis stained with Anthony's capsule stain. (Gary E. Kaiser, The Community College of Baltimore County, Catonsville campus, Baltimore, MD)
FIG. 3. Nonencapsulated Bacillus megaterium grown in skim milk broth and stained with Anthony's capsule stain. (Roxana B. Hughes, University of North Texas, Denton, TX)

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Figure 3
FIG. 3. Nonencapsulated Bacillus megaterium grown in skim milk broth and stained with Anthony's capsule stain. (Roxana B. Hughes, University of North Texas, Denton, TX)
FIG. 9. Nonencapsulated Staphylococus epidermidis grown on skim milk medium and stained using Maneval's method. (Ann C. Smith, University of Maryland, College Park, MD)

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Figure 4
FIG. 9. Nonencapsulated Staphylococus epidermidis grown on skim milk medium and stained using Maneval's method. (Ann C. Smith, University of Maryland, College Park, MD)
FIG. 6. Encapsulated Klebsiella pneumoniae grown in nutrient broth and stained using the Anthony's capsule stain. Note that no capsule is visible because no milk or other proteinaceous material was present or added to take up the crystal violet stain and provide a background.

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Figure 5
FIG. 6. Encapsulated Klebsiella pneumoniae grown in nutrient broth and stained using the Anthony's capsule stain. Note that no capsule is visible because no milk or other proteinaceous material was present or added to take up the crystal violet stain and provide a background.
FIG. 7. Encapsulated Serratia marcescens grown in skim milk broth and stained with Anthony's capsule stain. (Roxana B. Hughes, University of North Texas, Denton, TX)

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Figure 6
FIG. 7. Encapsulated Serratia marcescens grown in skim milk broth and stained with Anthony's capsule stain. (Roxana B. Hughes, University of North Texas, Denton, TX)