Bacterial Agglutination Protocol

  • Author: D. Sue Katz 1
    Affiliations: 1: Biology Department, Rogers State University, Claremore, OK, 74017
  • Citation: D. Sue Katz. 2011. Bacterial agglutination protocol.
  • Publication Date : November 2011
  • Category: Protocol
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Figure 1

An illustration of the effect of antibody and antigen concentrations on the amount of antibody-antigen precipitate observed in an agglutination reaction. Precipitation occurs when the concentrations of antibody and antigen allow the formation of crosslinked networks, created when antibodies bind two or more antigen epitopes. Maximal precipitate is observed in the concentration range termed "zone of equivalence." In the prozone region, the concentration of antibody exceeds the concentration of antigen and little antibody-antigen precipitation will be formed. In the postzone region, the concentration of antigen exceeds the concentration of antibody, again, limiting the production of antibody-antigen precipitate. (D. Sue Katz, Rogers?? State University, Claremore, OK? )

Source: November 2011
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Figure 2

Positive and negative agglutination. (A) Positive agglutination of Salmonella by anti-Salmonella antibodies. ( B) No agglutination is observed when anti-Salmonella antibodies are mixed with Escherichia coli. (Anne Hanson, University of Maine, Orono??, ME? )

Source: November 2011
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