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EcoSal Plus

Domain 4:

Synthesis and Processing of Macromolecules

Transfer RNA Modification: Presence, Synthesis, and Function

MyBook is a cheap paperback edition of the original book and will be sold at uniform, low price.
  • Authors: Glenn R. Björk1, and Tord G. Hagervall2
  • Editor: Susan T. Lovett3
    Affiliations: 1: Department of Molecular Biology, Umeå University, S-90187 Umeå, Sweden; 2: Department of Molecular Biology, Umeå University, S-90187 Umeå, Sweden; 3: Department of Biology, Brandeis University, Waltham, MA
  • Received 03 February 2014 Accepted 11 April 2014 Published 01 August 2014
  • Address correspondence to Glenn R. Björk, [email protected]
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  • Abstract:

    Transfer RNA (tRNA) from all organisms on this planet contains modified nucleosides, which are derivatives of the four major nucleosides. tRNA from / serovar Typhimurium contains 33 different modified nucleosides, which are all, except one (Queuosine [Q]), synthesized on an oligonucleotide precursor, which by specific enzymes later matures into tRNA. The structural genes for these enzymes are found in mono- and polycistronic operons, the latter of which have a complex transcription and translation pattern. The synthesis of the tRNA-modifying enzymes is not regulated similarly, and it is not coordinated to that of their substrate, the tRNA. The synthesis of some of them (e.g., several methylated derivatives) is catalyzed by one enzyme, which is position and base specific, whereas synthesis of some has a very complex biosynthetic pathway involving several enzymes (e.g., 2-thiouridines, -cyclicthreonyladenosine [ctA], and Q). Several of the modified nucleosides are essential for viability (e.g., lysidin, ctA, 1-methylguanosine), whereas the deficiency of others induces severe growth defects. However, some have no or only a small effect on growth at laboratory conditions. Modified nucleosides that are present in the anticodon loop or stem have a fundamental influence on the efficiency of charging the tRNA, reading cognate codons, and preventing missense and frameshift errors. Those that are present in the body of the tRNA primarily have a stabilizing effect on the tRNA. Thus, the ubiquitous presence of these modified nucleosides plays a pivotal role in the function of the tRNA by their influence on the stability and activity of the tRNA.

  • Citation: Björk G, Hagervall T. 2014. Transfer RNA Modification: Presence, Synthesis, and Function, EcoSal Plus 2014; doi:10.1128/ecosalplus.ESP-0007-2013

Article Version

This article is an updated version of the following content:


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Transfer RNA (tRNA) from all organisms on this planet contains modified nucleosides, which are derivatives of the four major nucleosides. tRNA from / serovar Typhimurium contains 33 different modified nucleosides, which are all, except one (Queuosine [Q]), synthesized on an oligonucleotide precursor, which by specific enzymes later matures into tRNA. The structural genes for these enzymes are found in mono- and polycistronic operons, the latter of which have a complex transcription and translation pattern. The synthesis of the tRNA-modifying enzymes is not regulated similarly, and it is not coordinated to that of their substrate, the tRNA. The synthesis of some of them (e.g., several methylated derivatives) is catalyzed by one enzyme, which is position and base specific, whereas synthesis of some has a very complex biosynthetic pathway involving several enzymes (e.g., 2-thiouridines, -cyclicthreonyladenosine [ctA], and Q). Several of the modified nucleosides are essential for viability (e.g., lysidin, ctA, 1-methylguanosine), whereas the deficiency of others induces severe growth defects. However, some have no or only a small effect on growth at laboratory conditions. Modified nucleosides that are present in the anticodon loop or stem have a fundamental influence on the efficiency of charging the tRNA, reading cognate codons, and preventing missense and frameshift errors. Those that are present in the body of the tRNA primarily have a stabilizing effect on the tRNA. Thus, the ubiquitous presence of these modified nucleosides plays a pivotal role in the function of the tRNA by their influence on the stability and activity of the tRNA.

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Structures of some modified nucleosides and their abbreviations. doi:10.1128/ecosalplus.ESP-0007-2013.f1

Citation: Björk G, Hagervall T. 2014. Transfer RNA Modification: Presence, Synthesis, and Function, EcoSal Plus 2014; doi:10.1128/ecosalplus.ESP-0007-2013
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Data are compiled from the Sprinzl database (http://trnadb.bioinf.uni-leipzig.de/ [ 17 ]) and the Modomics database (http://modomics.genesilico.pl/). Yellow positio