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EcoSal Plus

Domain 7:

Genetics and Genetic Tools

Prokaryotic Organelles: Bacterial Microcompartments in and

MyBook is a cheap paperback edition of the original book and will be sold at uniform, low price.
  • Authors: Katie L. Stewart1, Andrew M. Stewart2, and Thomas A. Bobik3
  • Editors: James M. Slauch4, Gregory Phillips5
  • VIEW AFFILIATIONS HIDE AFFILIATIONS
    Affiliations: 1: The Roy J. Carver Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, IA, USA 50011; 2: The Roy J. Carver Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, IA, USA 50011; 3: The Roy J. Carver Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, IA, USA 50011; 4: The School of Molecular and Cellular Biology, University of Illinois at Urbana-Champaign, Urbana, IL; 5: College of Veterinary Medicine, Iowa State University, Ames, IA
  • Received 07 May 2020 Accepted 04 September 2020 Published 06 October 2020
  • Address correspondence to Thomas A. Bobik, [email protected]
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  • Abstract:

    Bacterial microcompartments (MCPs) are proteinaceous organelles consisting of a metabolic pathway encapsulated within a selectively permeable protein shell. Hundreds of species of bacteria produce MCPs of at least nine different types, and MCP metabolism is associated with enteric pathogenesis, cancer, and heart disease. This review focuses chiefly on the four types of catabolic MCPs (metabolosomes) found in and : the propanediol utilization (), ethanolamine utilization (), choline utilization (), and glycyl radical propanediol () MCPs. Although the great majority of work done on catabolic MCPs has been carried out with and , research outside the group is mentioned where necessary for a comprehensive understanding. Salient characteristics found across MCPs are discussed, including enzymatic reactions and shell composition, with particular attention paid to key differences between classes of MCPs. We also highlight relevant research on the dynamic processes of MCP assembly, protein targeting, and the mechanisms that underlie selective permeability. Lastly, we discuss emerging biotechnology applications based on MCP principles and point out challenges, unanswered questions, and future directions.

  • Citation: Stewart K, Stewart A, Bobik T. 2020. Prokaryotic Organelles: Bacterial Microcompartments in and , EcoSal Plus 2020; doi:10.1128/ecosalplus.ESP-0025-2019

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/content/journal/ecosalplus/10.1128/ecosalplus.ESP-0025-2019
2020-10-06
2020-10-27

Abstract:

Bacterial microcompartments (MCPs) are proteinaceous organelles consisting of a metabolic pathway encapsulated within a selectively permeable protein shell. Hundreds of species of bacteria produce MCPs of at least nine different types, and MCP metabolism is associated with enteric pathogenesis, cancer, and heart disease. This review focuses chiefly on the four types of catabolic MCPs (metabolosomes) found in and : the propanediol utilization (), ethanolamine utilization (), choline utilization (), and glycyl radical propanediol () MCPs. Although the great majority of work done on catabolic MCPs has been carried out with and , research outside the group is mentioned where necessary for a comprehensive understanding. Salient characteristics found across MCPs are discussed, including enzymatic reactions and shell composition, with particular attention paid to key differences between classes of MCPs. We also highlight relevant research on the dynamic processes of MCP assembly, protein targeting, and the mechanisms that underlie selective permeability. Lastly, we discuss emerging biotechnology applications based on MCP principles and point out challenges, unanswered questions, and future directions.

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Figures

Image of Figure 1
Figure 1

Representative operons from LT2 (, ), 536 (), and CFT073 () are shown using one-letter abbreviations for genes and protein products. Gene sizes and spacing are roughly to scale. Note that in the operon, shell proteins are named with a different acronym, to - to avoid redundancy with prior work.

Citation: Stewart K, Stewart A, Bobik T. 2020. Prokaryotic Organelles: Bacterial Microcompartments in and , EcoSal Plus 2020; doi:10.1128/ecosalplus.ESP-0025-2019
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Figure 2

(A) Pdu MCP; (B) Eut MCP; (C) Cut MCP; (D) Grp MCP. Shell protein names are shown in brown, signature enzymes in green, accessory enzymes (where applicable) in light green, aldehyde dehydrogenase in purple, alcohol dehydrogenase in red, phosphotransacetylase in blue, and acetate kinase in gold.

Citation: Stewart K, Stewart A, Bobik T. 2020. Prokaryotic Organelles: Bacterial Microcompartments in and , EcoSal Plus 2020; doi:10.1128/ecosalplus.ESP-0025-2019
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Figure 3

(A) Cut MCPs from 536; (B) Grp MCPs from CFT073; (C) Pdu MCPs from . Scale bars are located on each image. Images were obtained as described ( 76 ).

Citation: Stewart K, Stewart A, Bobik T. 2020. Prokaryotic Organelles: Bacterial Microcompartments in and , EcoSal Plus 2020; doi:10.1128/ecosalplus.ESP-0025-2019
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Figure 4

(A) PduA BMC-H protein from (PDB ID: 3NGK) ( 155 ); (B) EutM, the orthologous BMC-H protein from K-12 (3I6P) ( 159 ); (C) PduU permuted BMC-H protein from serovar Typhimurium (3CGI) ( 154 ); (D) EutL BMC-T closed form from K12 (3GFH) ( 160 ); (E) EutL BMC-T open form from K12 (3I87) ( 159 ); (F) PduT [Fe-S]-containing BMC-T protein from (3PAC) [ 156 ]; (G) GrpN BMV pentamer from (4I7A) ( 158 ).

Citation: Stewart K, Stewart A, Bobik T. 2020. Prokaryotic Organelles: Bacterial Microcompartments in and , EcoSal Plus 2020; doi:10.1128/ecosalplus.ESP-0025-2019
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Tables

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TABLE 1

MCP operons found in and , taken from genomic studies ( 4 )

Citation: Stewart K, Stewart A, Bobik T. 2020. Prokaryotic Organelles: Bacterial Microcompartments in and , EcoSal Plus 2020; doi:10.1128/ecosalplus.ESP-0025-2019

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