Identification of an Alternative to Proteus vulgaris as a Laboratory Standard for Hydrogen Sulfide Production
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Authors:
Nar’Asha Randall1,
Cara Nance1,
Cathy Huggins1,
James Huggins1,
Mark Bolyard1,*
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Received 08 August 2016 Accepted 09 November 2016 Published 21 April 2017
- ©2017 Author(s). Published by the American Society for Microbiology.
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[open-access] This is an Open Access article distributed under the terms of the Creative Commons Attribution-Noncommercial-NoDerivatives 4.0 International license (https://creativecommons.org/licenses/by-nc-nd/4.0/ and https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode), which grants the public the nonexclusive right to copy, distribute, or display the published work.
- *Corresponding author. Mailing address: Biology Department, Union University, 1050 Union University Drive, Box 3021, Jackson, TN 38305. Phone: 731-661-6586. E-mail: [email protected].
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Affiliations:
1: Department of Biology, Union University, Jackson, TN 38305
AUTHOR AND ARTICLE INFORMATION
AUTHOR AND ARTICLE INFORMATION
Source:
J. Microbiol. Biol. Educ.
April 2017
vol. 18 no. 1 doi:10.1128/jmbe.v18i1.1219
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Abstract:
This project involved the evaluation of a biosafety level 1 alternative to Proteus vulgaris as a positive control for the production of hydrogen sulfide. We determined that Citrobacter freundii could serve as an excellent substitute for P. vulgaris, and that lead acetate strips used in conjunction with triple sugar iron media allows for consistent results following evaluation after up to one week.
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2017-04-21
2021-04-15
Abstract:
This project involved the evaluation of a biosafety level 1 alternative to Proteus vulgaris as a positive control for the production of hydrogen sulfide. We determined that Citrobacter freundii could serve as an excellent substitute for P. vulgaris, and that lead acetate strips used in conjunction with triple sugar iron media allows for consistent results following evaluation after up to one week.
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Author and Article Information
-
Received 08 August 2016 Accepted 09 November 2016 Published 21 April 2017
- ©2017 Author(s). Published by the American Society for Microbiology.
-
[open-access] This is an Open Access article distributed under the terms of the Creative Commons Attribution-Noncommercial-NoDerivatives 4.0 International license (https://creativecommons.org/licenses/by-nc-nd/4.0/ and https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode), which grants the public the nonexclusive right to copy, distribute, or display the published work.
- *Corresponding author. Mailing address: Biology Department, Union University, 1050 Union University Drive, Box 3021, Jackson, TN 38305. Phone: 731-661-6586. E-mail: [email protected].
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Identification of an Alternative to Proteus vulgaris as a Laboratory Standard for Hydrogen Sulfide Production
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Citation:
Randall N, Nance C, Huggins C, Huggins J, Bolyard M. 2017. Identification of an alternative to proteus vulgaris as a laboratory standard for hydrogen sulfide production. J. Microbiol. Biol. Educ. 18(1): doi:10.1128/jmbe.v18i1.1219
/content/jmbe/10.1128/jmbe.v18i1.1219.f1-jmbe-18-20
FIGURE 1
Lead acetate strips from C. freundii, P. vulgaris, and E. aerogenes from a 48-hour stab culture using triple sugar iron (TSI) or peptone iron (PI).
jmbe/18/1/jmbe-18-20f1_thmb.gif
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FIGURE 1
Lead acetate strips from C. freundii, P. vulgaris, and E. aerogenes from a 48-hour stab culture using triple sugar iron (TSI) or peptone iron (PI).
Source:
J. Microbiol. Biol. Educ.
April 2017
vol. 18 no. 1 doi:10.1128/jmbe.v18i1.1219
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