Bacterial DNA Extraction Using Individual Enzymes and Phenol/Chloroform Separation †
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Authors:
Mitchell Henry Wright1,*,
Joseph Adelskov2,
Anthony Carlson Greene2
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Received 02 May 2017 Accepted 03 July 2017 Published 01 September 2017
- ©2017 Author(s). Published by the American Society for Microbiology
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[open-access] This is an Open Access article distributed under the terms of the Creative Commons Attribution-Noncommercial-NoDerivatives 4.0 International license (https://creativecommons.org/licenses/by-nc-nd/4.0/ and https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode), which grants the public the nonexclusive right to copy, distribute, or display the published work.
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†Supplemental materials available at http://asmscience.org/jmbe
- *Corresponding author. Mailing address: Division of Environmental and Biomolecular Systems, Institute of Environmental Health, Oregon Health & Science University, 3181 S.W. Sam Jackson Park Rd., Portland, OR, 97239-3098. Phone: 503-346-3434. E-mail: [email protected].
Abstract:
Marmur (4) developed one of the first detailed comprehensive methods for purifying bacterial DNA. This procedure is now outdated, and can be difficult to follow for those with limited experience in molecular biology. Here, we provide a modernized, simplified protocol for extracting bacterial DNA and discuss how this can be incorporated into microbiology laboratory courses for biology majors.
References & Citations
Supplemental Material
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Appendix 1: Pre-laboratory questions, Appendix 2: Pre-laboratory answer key, Appendix 3: DNA extraction flowchart, Appendix 4: Agarose gel preparation for electrophoresis
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Abstract:
Marmur (4) developed one of the first detailed comprehensive methods for purifying bacterial DNA. This procedure is now outdated, and can be difficult to follow for those with limited experience in molecular biology. Here, we provide a modernized, simplified protocol for extracting bacterial DNA and discuss how this can be incorporated into microbiology laboratory courses for biology majors.

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Author and Article Information
-
Received 02 May 2017 Accepted 03 July 2017 Published 01 September 2017
- ©2017 Author(s). Published by the American Society for Microbiology
-
[open-access] This is an Open Access article distributed under the terms of the Creative Commons Attribution-Noncommercial-NoDerivatives 4.0 International license (https://creativecommons.org/licenses/by-nc-nd/4.0/ and https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode), which grants the public the nonexclusive right to copy, distribute, or display the published work.
-
†Supplemental materials available at http://asmscience.org/jmbe
- *Corresponding author. Mailing address: Division of Environmental and Biomolecular Systems, Institute of Environmental Health, Oregon Health & Science University, 3181 S.W. Sam Jackson Park Rd., Portland, OR, 97239-3098. Phone: 503-346-3434. E-mail: [email protected].
Figures
Agarose gel containing λHind III linear standard (A) and purified Escherichia coli genomic DNA (B, C). Visualized under UV light.

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FIGURE 1
Agarose gel containing λHind III linear standard (A) and purified Escherichia coli genomic DNA (B, C). Visualized under UV light.