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Elementary Student Outreach Activity Demonstrating the Use of Phage Therapy Heroes to Combat Bacterial Infections

    Authors: Mya Breitbart1,*, Kema Malki1, Natalie A. Sawaya1, Chelsea Bonnain1, Mark O. Martin2
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    Affiliations: 1: University of South Florida, Saint Petersburg, FL 33701; 2: University of Puget Sound, Tacoma, WA 98416
    AUTHOR AND ARTICLE INFORMATION AUTHOR AND ARTICLE INFORMATION
    Source: J. Microbiol. Biol. Educ. March 2018 vol. 19 no. 1 doi:10.1128/jmbe.v19i1.1407
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References & Citations

1. Human Microbiome Project Consortium 2012 Structure, function and diversity of the healthy human microbiome Nature 486 207 214 10.1038/nature11234 22699609 3564958 http://dx.doi.org/10.1038/nature11234
2. Parvez S, Malik KA, Ah Kang S, Kim HY 2006 Probiotics and their fermented food products are beneficial for health J Appl Microbiol 100 1171 1185 10.1111/j.1365-2672.2006.02963.x 16696665 http://dx.doi.org/10.1111/j.1365-2672.2006.02963.x
3. Fauci AS, Morens DM 2016 Zika virus in the Americas—yet another arbovirus threat New Engl J Med 374 601 604 10.1056/NEJMp1600297 http://dx.doi.org/10.1056/NEJMp1600297
4. Roossinck MJ 2015 Move over, bacteria! Viruses make their mark as mutualistic microbial symbionts J Virol 89 6532 6535 10.1128/JVI.02974-14 25903335 4468468 http://dx.doi.org/10.1128/JVI.02974-14
5. Kutter E, De Vos D, Gvasalia G, Alavidze Z, Gogokhia L, Kuhl S, Abedon ST 2010 Phage therapy in clinical practice: treatment of human infections Curr Pharm Biotech 11 69 86 10.2174/138920110790725401 http://dx.doi.org/10.2174/138920110790725401
6. Burrowes B, Harper DR, Anderson J, McConville M, Enright MC 2011 Bacteriophage therapy: potential uses in the control of antibiotic-resistant pathogens Expert Rev Anti Infect Ther 9 775 785 10.1586/eri.11.90 21905786 http://dx.doi.org/10.1586/eri.11.90
7. Kropinski AM, Mazzocco A, Waddell TE, Lingohr E, Johnson RP 2009 Enumeration of bacteriophages by double agar overlay plaque assay Methods Mol Biol 501 69 76 10.1007/978-1-60327-164-6_7 http://dx.doi.org/10.1007/978-1-60327-164-6_7
8. Capparelli R, Parlato M, Borriello G, Salvatore P, Iannelli D 2007 Experimental phage therapy against Staphylococcus aureus in mice Antimicrob Agents Chem 51 2765 2773 10.1128/AAC.01513-06 http://dx.doi.org/10.1128/AAC.01513-06
9. Efrony R, Loya Y, Bacharach E, Rosenberg E 2007 Phage therapy of coral disease Coral Reefs 26 7 13 10.1007/s00338-006-0170-1 http://dx.doi.org/10.1007/s00338-006-0170-1
10. Guenther S, Huwyler D, Richard S, Loessner MJ 2009 Virulent bacteriophage for efficient biocontrol of Listeria monocytogenes in ready-to-eat foods Appl Environ Microbiol 75 93 100 10.1128/AEM.01711-08 2612219 http://dx.doi.org/10.1128/AEM.01711-08
11. Endersen L, O’Mahony J, Hill C, Ross RP, McAuliffe O, Coffey A 2014 Phage therapy in the food industry Ann Rev Food Sci Technol 5 327 249 10.1146/annurev-food-030713-092415 http://dx.doi.org/10.1146/annurev-food-030713-092415

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2018-03-30
2019-09-15
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Figures

Image of FIGURE 1

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FIGURE 1

The team of phage heroes conquering their bacterial villains (artwork by Kema Malki). This design can be used to make custom temporary tattoos to serve as a reward for successfully capturing the correct bacteria in the outreach activity.

Source: J. Microbiol. Biol. Educ. March 2018 vol. 19 no. 1 doi:10.1128/jmbe.v19i1.1407
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Image of FIGURE 2

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FIGURE 2

Example illustrating the set-up of the outreach activity. The diameter of the hula hoop in this photo is ~1 meter with all heroes and villains printed full-scale from the appendices. This activity can be modified for smaller learning spaces by using a paper plate instead of a hula-hoop and reducing the size of the bacterial colonies and phage heroes accordingly.

Source: J. Microbiol. Biol. Educ. March 2018 vol. 19 no. 1 doi:10.1128/jmbe.v19i1.1407
Download as Powerpoint
Image of FIGURE 3

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FIGURE 3

Plaque assay performed according to standard double-agar overlay methods ( 7 ) using phage T4 and four different strains of chromogenic bacteria to illustrate the clear plaques (zones of death) caused by phages in the bacterial lawns of different colors.

Source: J. Microbiol. Biol. Educ. March 2018 vol. 19 no. 1 doi:10.1128/jmbe.v19i1.1407
Download as Powerpoint

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