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A Simple and Effective Method for Extracting Potential Mutagens from Sediment Samples in the Classroom Laboratory Setting

    Authors: Catalina Durán1, Valentina Blanco1, Claudia Piccini2, Pablo Zunino2, Eliana Rodríguez1,*
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    Affiliations: 1: Unidad Académica de Laboratorios Prácticos, Facultad de Ciencias, Universidad de la República, Uruguay; 2: Departamento de Microbiología, Instituto de Investigaciones Biológicas Clemente Estable, Uruguay
    AUTHOR AND ARTICLE INFORMATION AUTHOR AND ARTICLE INFORMATION
    • Received 19 October 2017 Accepted 31 January 2018 Published 27 April 2018
    • ©2018 Author(s). Published by the American Society for Microbiology.
    • [open-access] This is an Open Access article distributed under the terms of the Creative Commons Attribution-Noncommercial-NoDerivatives 4.0 International license (https://creativecommons.org/licenses/by-nc-nd/4.0/ and https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode), which grants the public the nonexclusive right to copy, distribute, or display the published work.

    • *Corresponding author. Mailing address: Unidad Académica de Laboratorios Prácticos, Facultad de Ciencias, Universidad de la República, Uruguay, Iguá 4225, 11400, Montevideo, Uruguay. Phone: 598-2525-8618, ext. 7229. E-mail: [email protected].
    Source: J. Microbiol. Biol. Educ. April 2018 vol. 19 no. 1 doi:10.1128/jmbe.v19i1.1509
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    Abstract:

    We describe a simple and effective method for the extraction of potential mutagens from sediment samples. This method is straightforward and does not require expensive laboratory equipment, thus enabling instructors to analyze the presence of mutagenic substances in a conventional classroom setting. Additionally, we found that students felt encouraged to add the Ames test to the list of analyses they traditionally employ with sediment samples. This link between the environment and the Ames test provided an authentic learning context for students, bridging the gap between the "real-world" and the classroom laboratory, and thus making the educational experience more engaging and meaningful.

References & Citations

1. Maron DM, Ames BN 1983 Revised methods for the Salmonella mutagenicity test Mutat Res 113 173 215 10.1016/0165-1161(83)90010-9 6341825 http://dx.doi.org/10.1016/0165-1161(83)90010-9
2. Rodríguez E, Piccini C, Sosa V, Zunino P 2012 The use of the Ames test as a tool for addressing problem-based learning in the microbiology lab J Microbiol Biol Educ 13 2 175 177 10.1128/jmbe.v13i2.421 23653807 3577329 http://dx.doi.org/10.1128/jmbe.v13i2.421
3. Hayden Galindo J 2014 Authentic learning (simulations, lab, field) Harvard University [Online.] https://ablconnect.harvard.edu/authentic-learning
4. Muniz P, Venturini N, Martínez A 2002 Physico-chemical characteristics and pollutants of the benthic environment in the Montevideo coastal zone, Uruguay Mar Pollut Bull 44 956 976 10.1016/S0025-326X(02)00111-X http://dx.doi.org/10.1016/S0025-326X(02)00111-X
5. Muniz P, Danulat E, Yannicelli B, García-Alonso J, Medina G, Bícego MC 2004 Assessment of contamination by heavy metals and petroleum hydrocarbons in sediments of Montevideo Harbour (Uruguay) Environ Int 29 1019 1028 10.1016/S0160-4120(03)00096-5 http://dx.doi.org/10.1016/S0160-4120(03)00096-5
6. Sueiro RA, Garrido MJ, Araujo M 2011 Mutagenic assessment of Prestige fuel oil spilled on the shore and submitted to field trials of bioremediation Sci Tot Environ 409 23 4973 4978 10.1016/j.scitotenv.2011.08.017 http://dx.doi.org/10.1016/j.scitotenv.2011.08.017
7. Vincent-Hubert F, Heas-Moisan K, Munschy C, Tronczynski J 2012 Mutagenicity and genotoxicity of suspended particulate matter in the Seine river estuary Mutat Res 741 1–2 7 12 10.1016/j.mrgentox.2011.09.019 http://dx.doi.org/10.1016/j.mrgentox.2011.09.019
8. Hafner C, Gartiser S, Garcia-Käufer M, Schiwy S, Hercher C, Meyer W, Achten C, Larsson M, Engwall M, Keiter S, Hollert H 2015 Investigations on sediment toxicity of German rivers applying a standardized bioassay battery Environ Sci Pollut Res 22 21 16358 16370 10.1007/s11356-015-4482-y http://dx.doi.org/10.1007/s11356-015-4482-y
9. Mortelmans K, Zeiger E 2000 The Ames Salmonella/microsome mutagenicity assay Mutat Res 455 1–2 29 60 10.1016/S0027-5107(00)00064-6 11113466 http://dx.doi.org/10.1016/S0027-5107(00)00064-6
10. Gatehouse D, Haworth S, Cebula T, Gocke E, Kier L, Matsushima T, Melcion C, Nohmi T, Ohta T, Venitt S, Zeiger E 1994 Recommendations for the performance of bacterial mutation assays Mutat Res 312 217 233 10.1016/0165-1161(94)90037-X http://dx.doi.org/10.1016/0165-1161(94)90037-X

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/content/journal/jmbe/10.1128/jmbe.v19i1.1509
2018-04-27
2019-10-16

Abstract:

We describe a simple and effective method for the extraction of potential mutagens from sediment samples. This method is straightforward and does not require expensive laboratory equipment, thus enabling instructors to analyze the presence of mutagenic substances in a conventional classroom setting. Additionally, we found that students felt encouraged to add the Ames test to the list of analyses they traditionally employ with sediment samples. This link between the environment and the Ames test provided an authentic learning context for students, bridging the gap between the "real-world" and the classroom laboratory, and thus making the educational experience more engaging and meaningful.

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