Student-Designed High-Throughput Assays to Assess Effects of Growth Insults in Budding Yeast †
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Authors:
Veronica A. Segarra1,*,
Carrie Wilson1,
Kelley Lowery1,
Slade Ransdell1,
Jamie Schnuck2,
Paul Way2,
Melissa C. Srougi2,*
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Received 29 November 2017 Accepted 23 April 2018 Published 29 June 2018
- ©2018 Author(s). Published by the American Society for Microbiology.
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[open-access] This is an Open Access article distributed under the terms of the Creative Commons Attribution-Noncommercial-NoDerivatives 4.0 International license (https://creativecommons.org/licenses/by-nc-nd/4.0/ and https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode), which grants the public the nonexclusive right to copy, distribute, or display the published work.
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†Supplemental materials available at http://asmscience.org/jmbe
- *Corresponding authors. Veronica A. Segarra. Mailing address: High Point University, 237 B Couch Hall, One University Parkway, High Point, NC 27268. Phone: 336-841-9507. Fax: 336-888-6341. E-mail: [email protected]. Melissa C. Srougi. Mailing address: High Point University, 355 D Couch Hall, One University Parkway, High Point, NC 27268. Phone: 336-841-9619. Fax: 336-888-6341. E-mail: [email protected].
Abstract:
Modern high-throughput screening is a versatile method used widely in academic and industrial labs for genetic and pharmacological testing, but is underutilized in undergraduate laboratories. We designed an open inquiry-based laboratory activity exploiting a small-scale high-throughput screening assay of the budding yeast S. cerevisiae to promote active learning and introduce students to modern data collection and analysis techniques. In this activity, students at both the high school and university levels generate growth curves of S. cerevisiae using a microplate reader after treatment with their insult of choice. This lab presents an ideal opportunity for both high school and university students to actively learn about the process of science as well as high-throughput data collection and analysis in a cost-effective manner.
References & Citations
Supplemental Material
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Appendix 1: Teacher materials and preparation notes for HTS yeast-growth experiments, Appendix 2: Student handout/protocol—Assessing the effects of microwave radiation on yeast growth, Appendix 3: Student handout/protocol—Assessing the effects of UV radiation on yeast growth, Appendix 4: Student handout/protocol—Assessing the effects of rapamycin on yeast growth
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Abstract:
Modern high-throughput screening is a versatile method used widely in academic and industrial labs for genetic and pharmacological testing, but is underutilized in undergraduate laboratories. We designed an open inquiry-based laboratory activity exploiting a small-scale high-throughput screening assay of the budding yeast S. cerevisiae to promote active learning and introduce students to modern data collection and analysis techniques. In this activity, students at both the high school and university levels generate growth curves of S. cerevisiae using a microplate reader after treatment with their insult of choice. This lab presents an ideal opportunity for both high school and university students to actively learn about the process of science as well as high-throughput data collection and analysis in a cost-effective manner.

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Author and Article Information
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Received 29 November 2017 Accepted 23 April 2018 Published 29 June 2018
- ©2018 Author(s). Published by the American Society for Microbiology.
-
[open-access] This is an Open Access article distributed under the terms of the Creative Commons Attribution-Noncommercial-NoDerivatives 4.0 International license (https://creativecommons.org/licenses/by-nc-nd/4.0/ and https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode), which grants the public the nonexclusive right to copy, distribute, or display the published work.
-
†Supplemental materials available at http://asmscience.org/jmbe
- *Corresponding authors. Veronica A. Segarra. Mailing address: High Point University, 237 B Couch Hall, One University Parkway, High Point, NC 27268. Phone: 336-841-9507. Fax: 336-888-6341. E-mail: [email protected]. Melissa C. Srougi. Mailing address: High Point University, 355 D Couch Hall, One University Parkway, High Point, NC 27268. Phone: 336-841-9619. Fax: 336-888-6341. E-mail: [email protected].
Figures

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FIGURE 1
Various insults decrease yeast growth. (A) Growth curves of S. cerevisiae treated for various times with microwave radiation. (B) Representative growth curves of wild-type S. cerevisiae and the mutant UV-sensitive strain treated with or without sunlight for various times. (C) Growth curves of S. cerevisiae treated for various times with 0.05 μg/mL, 0.1 μg/mL or 0.2 μg/mL of rapamycin. Results are representative of university students’ data. Averages ± STD of experiments performed in triplicate are shown