A Microbial Sampling and Community Reconstruction Activity for Introducing Students to the Burgeoning Field of Metagenomics

    Authors: Ashley N. Williams1, John Stavrinides1,*
    Affiliations: 1: Department of Biology, University of Regina, Regina, SK S4S 0A2, Canada
    • Received 08 August 2019 Accepted 27 January 2020 Published 30 April 2020
    • ©2020 Author(s). Published by the American Society for Microbiology
    • [open-access] This is an Open Access article distributed under the terms of the Creative Commons Attribution-Noncommercial-NoDerivatives 4.0 International license (https://creativecommons.org/licenses/by-nc-nd/4.0/ and https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode), which grants the public the nonexclusive right to copy, distribute, or display the published work.

    • Supplemental materials available at http://asmscience.org/jmbe
    • *Corresponding author. Mailing address: Department of Biology, University of Regina, 3737 Wascana Parkway, Regina, SK S4S 0A2, Canada. Phone: 306-337-8478. E-mail: [email protected].
    Source: J. Microbiol. Biol. Educ. April 2020 vol. 21 no. 1 doi:10.1128/jmbe.v21i1.1891
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References & Citations

1. Martiny AC 2019 High proportions of bacteria are culturable across major biomes ISME J 13 2125 2128 10.1038/s41396-019-0410-3 30952994 6775996 http://dx.doi.org/10.1038/s41396-019-0410-3
2. Rappé MS, Giovannoni SJ 2003 The uncultured microbial majority Ann Rev Microbiol 57 369 394 10.1146/annurev.micro.57.030502.090759 http://dx.doi.org/10.1146/annurev.micro.57.030502.090759
3. Jurkowski A, Reid AH, Labov JB 2007 Metagenomics: a call for bringing a new science into the classroom (while it’s still new) CBE Life Sci Educ 6 260 265 10.1187/cbe.07-09-0075 18056294 2104496 http://dx.doi.org/10.1187/cbe.07-09-0075
4. Garza DR, Dutilh BE 2015 From cultured to uncultured genome sequences: metagenomics and modeling microbial ecosystems Cell Mol Life Sci 72 4287 4308 10.1007/s00018-015-2004-1 26254872 4611022 http://dx.doi.org/10.1007/s00018-015-2004-1
5. Hugerth LW, Andersson AF 2017 Analysing microbial community composition through amplicon sequencing: from sampling to hypothesis testing Front Microbiol 8 1561 10.3389/fmicb.2017.01561 28928718 5591341 http://dx.doi.org/10.3389/fmicb.2017.01561

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