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Human Immunodeficiency Virus

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  • Authors: Wendy S. Armstrong1, Jeannette Guarner2, Colleen S. Kraft3, Angela M. Caliendo4
  • Editors: Randall T. Hayden5, Donna M. Wolk6, Karen C. Carroll7, Yi-Wei Tang8
  • VIEW AFFILIATIONS HIDE AFFILIATIONS
    Affiliations: 1: Division of Infectious Diseases, Emory University, Atlanta, GA 30322; 2: Division of Infectious Diseases, Department of Pathology and Laboratory Medicine, Emory University, Atlanta, GA 30322; 3: Division of Infectious Diseases, Department of Pathology and Laboratory Medicine, Emory University, Atlanta, GA 30322; 4: Department of Medicine, Alpert Medical School Brown University, Providence, RI 02903; 5: St. Jude’s Children’s Research Hospital, Memphis, TN; 6: Geisinger Clinic, Danville, PA; 7: Johns Hopkins University Hospital, Baltimore, MD; 8: Memorial Sloane-Kettering Institute, New York, NY
    • Source: microbiolspec July 2016 vol. 4 no. 4 doi:10.1128/microbiolspec.DMIH2-0024-2015
  • Received 05 October 2015 Accepted 01 February 2016 Published 01 July 2016
  • Angela Caliendo, [email protected]
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  • Abstract:

    In this chapter we will discuss the diagnosis and monitoring of individuals with HIV infection. The application and interpretation of these tests does not change dramatically when used in the immunocompromised host. The principal approach to the diagnosis of HIV infection involves serologic testing, although nucleic acid amplification tests play an important role in the diagnosis of acute HIV infection. The algorithm for diagnosis of HIV continues to evolve with newer assays that are able to detect infection within an earlier timeframe after HIV transmission. Viral load testing for HIV-1 is the cornerstone for monitoring patients on antiretroviral therapy. Genotypic and phenotypic resistance tests are employed when antiretroviral resistance is suspected and results help guide therapy. The tropism assay must be performed to determine the efficacy of CCR5 chemokine receptor antagonists. Next-generation sequencing methods are an innovative approach to assessing archived antiretroviral resistance in patients with virologic suppression. The success of antiretroviral therapy with improved long-term outcomes has made transplantation in HIV-infected patients a reality.

  • Citation: Armstrong W, Guarner J, Kraft C, Caliendo A. 2016. Human Immunodeficiency Virus. Microbiol Spectrum 4(4):DMIH2-0024-2015. doi:10.1128/microbiolspec.DMIH2-0024-2015.

References

1. Gao F, Bailes E, Robertson DL, Chen Y, Rodenburg CM, Michael SF, Cummins LB, Arthur LO, Peeters M, Shaw GM, Sharp PM, Hahn BH. 1999. Origin of HIV-1 in the chimpanzee Pan troglodytes troglodytes. Nature 397:436–441. [CrossRef]
2. Sharp PM, Bailes E, Chaudhuri RR, Rodenburg CM, Santiago MO, Hahn BH. 2001. The origins of acquired immune deficiency syndrome viruses: where and when? Philos Trans R Soc Lond B Biol Sci 356:867–876. [CrossRef]
3. Hunter E. 2008. Retroviruses. p 680 In Mahy BWJ and van Regenmortel MHV (ed), Encyclopedia of Virology, 3rd ed. Elsevier, Amsterdam. [CrossRef]
4. Ronen K, Sharma A, Overbaugh J. 2015. HIV transmission biology: translation for HIV prevention. AIDS 29:2219–2227. [CrossRef]
5. Patel P, Borkowf CB, Brooks JT, Lasry A, Lansky A, Mermin J. 2014. Estimating per-act HIV transmission risk: a systematic review. AIDS 28:1509–1519. [CrossRef]
6. Eshleman SH, Hudelson SE, Ou SS, Redd AD, Swanstrom R, Porcella SF, Chen YQ, Piwowar-Manning E, McCauley M, Gamble T, Sievers M, Martens CA, Bruno D, Ping L-H, Dukhovlinova E, Quinn TC, Kumwenda J, Madiwichi M, Nhando N, Akelo V, Moyo S, Panchia R, Kumarasamy N, Chotirosniramit N, Rocha MM, Bustorff F, Brinsztejn B, Mayer KH, Hughes JP, Cohen MS for the HPTN O52 Study Team. 2015. Treatment as prevention: characterization of partner infections in the HIV Prevention Trials Network 052 trial. Program number MOAC0106LB, 8th IAS Conference on HIV Pathogenesis, Treatment and Prevention, Vancouver, Canada.
7. Douek D. 2007. HIV disease progression: immune activation, microbes, and a leaky gut. Top HIV Med 15:114–117.
8. Mellors JW, Muñoz A, Giorgi JV, Margolick JB, Tassoni CJ, Gupta P, Kingsley LA, Todd JA, Saah AJ, Detels R, Phair JP, Rinaldo CR Jr. 1997. Plasma viral load and CD4 + lymphocytes as prognostic markers of HIV-1 infection. Ann Intern Med 126:946–954. [CrossRef]
9. Rodger AJ, Lodwick R, Schechter M, Deeks S, Amin J, Gilson R, Paredes R, Bakowska E, Engsig FN, Phillips A, Insight Smart ESG, INSIGHT SMART, ESPRIT Study Groups. 2013. Mortality in well controlled HIV in the continuous antiretroviral therapy arms of the SMART and ESPRIT trials compared with the general population. AIDS 27:973–979. [CrossRef]
10. Locke JE, Durand C, Reed RD, MacLennan PA, Mehta S, Massie A, Nellore A, DuBay D, Segev DL. 2016. Long-term outcomes after liver transplantation among human immunodeficiency virus-infected recipients. Transplantation 100:141–146. [CrossRef]
11. Locke JE, Mehta S, Reed RD, MacLennan P, Massie A, Nellore A, Durand C, Segev DL. 2015. A national study of outcomes among HIV-infected kidney transplant recipients. J Am Soc Nephrol 26:2222–2229. [CrossRef]
12. Stock PG, Barin B, Murphy B, Hanto D, Diego JM, Light J, Davis C, Blumberg E, Simon D, Subramanian A, Millis JM, Lyon GM, Brayman K, Slakey D, Shapiro R, Melancon J, Jacobson JM, Stosor V, Olson JL, Stablein DM, Roland ME. 2010. Outcomes of kidney transplantation in HIV-infected recipients. N Engl J Med 363:2004–2014. [CrossRef]
13. Muller E, Barday Z, Mendelson M, Kahn D. 2015. HIV-positive-to-HIV-positive kidney transplantation—results at 3 to 5 years. N Engl J Med 372:613–620. [CrossRef]
14. Hütter G, Nowak D, Mossner M, Ganepola S, Müssig A, Allers K, Schneider T, Hofmann J, Kücherer C, Blau O, Blau IW, Hofmann WK, Thiel E. 2009. Long-term control of HIV by CCR5 delta32/delta32 stem-cell transplantation. N Engl J Med 360:692–698. [CrossRef]
15. Henrich TJ, Hanhauser E, Marty FM, Sirignano MN, Keating S, Lee TH, Robles YP, Davis BT, Li JZ, Heisey A, Hill AL, Busch MP, Armand P, Soiffer RJ, Altfeld M, Kuritzkes DR. 2014. Antiretroviral-free HIV-1 remission and viral rebound after allogeneic stem cell transplantation: report of 2 cases. Ann Intern Med 161:319–327. [CrossRef]
16. Durand CM, Ambinder RF. 2013. Hematopoietic stem cell transplantation in HIV-1-infected individuals: clinical challenges and the potential for viral eradication. Curr Opin Oncol 25:180–186. [CrossRef]
17. Chappel RJ, Wilson KM, Dax EM. 2009. Immunoassays for the diagnosis of HIV: meeting future needs by enhancing the quality of testing. Future Microbiol 4:963–982. [CrossRef]
18. Masciotra S, McDougal J, Feldman J, Sprinkle P, Wesolowski L, Owen S. 2011. Evaluation of an alternative HIV diagnostic algorithm using specimens from seroconversion panels and persons with established HIV infections. J Clin Virol 52S:S17–S22. [CrossRef]
19. Branson B. 2015. HIV testing updates and challenges: when regulatory caution and public health imperatives collide. Curr HIV/AIDS Rep 12:117–126. [CrossRef]
20. Bio-Rad-Laboratories. 2011. Package insert—GS HIV Combo Ag/Ab EIA.
21. Alter HJ, Epstein JS, Swenson SG, VanRaden MJ, Ward JW, Kaslow RA, Menitove JE, Klein HG, Sandler SG, Sayers MH, Hewlett I, Chernoff A, The HIV-Antigen Study Group. 1990. Prevalence of human immunodeficiency virus type 1 p24 antigen in U.S. blood donors—an assessment of the efficacy of testing in donor screening. N Engl J Med 323:1312–1317. [CrossRef]
22. Jurriaans S, Back N, Wolthers K. 2011. Ten years of HIV testing with fourth-generation assays: the Amsterdam experience. J Clin Virol 52S:S67–S69. [CrossRef]
23. Centers for Disease Control and Prevention (CDC). 2013. Detection of acute HIV infection in two evaluations of a new HIV diagnostic testing algorithm - United States, 2011-2013. MMWR Morb Mortal Wkly Rep 62:489–494.
24. Masciotra S, Smith A, Youngpairoj A, Sprinkle P, Miles I, Sionean C, Paz-Bailey G, Johnson J, Owen S. 2013. Evaluation of the CDC-proposed laboratory HIV testing algorithm among men who have sex with men (MSM) from five US metropolitan statistical areas using specimens collected in 2011. J Clin Virol 58S:e8–e12. [CrossRef]
25. Fransen K, Vermoesen T, Beelaert G, Menten J, Hutse V, Wouters K, Platteau T, Florence E. 2013. Using conventional HIV tests on oral fluid. J Virol Methods 194:46–51. [CrossRef]
26. Pilcher CD, Louie B, Facente S, Keating S, Hackett J Jr, Vallari A, Hall C, Dowling T, Busch MP, Klausner JD, Hecht FM, Liska S, Pandori MW. 2013. Performance of rapid point-of-care and laboratory tests for acute and established HIV infection in San Francisco. PLoS One 8:e80629–e80637. [CrossRef]
27. Pant Pai N, Balram B, Shivkumar S, Martinez-Cajas JL, Claessens C, Lambert G, Peeling RW, Joseph L. 2012. Head-to-head comparison of accuracy of a rapid point-of-care HIV test with oral versus whole-blood specimens: a systematic review and meta-analysis. Lancet Infect Dis 12:373–380. [CrossRef]
28. Black V, von Mollendorf CE, Moyes JA, Scott LE, Puren A, Stevens WS. 2009. Poor sensitivity of field rapid HIV testing: implications for mother-to-child transmission programme. BJOG 116:1805–1808. [CrossRef]
29. Orgenics-Ltd. 2013. Alere Determine™ HIV–1/2 Ag/Ab Combo package insert.
30. Masciotra S, Luo W, Youngpairoj AS, Kennedy MS, Wells S, Ambrose K, Sprinkle P, Owen SM. 2013. Performance of the Alere Determine HIV-1/2 Ag/Ab Combo Rapid Test with specimens from HIV-1 seroconverters from the US and HIV-2 infected individuals from Ivory Coast. J Clin Virol 58(Suppl 1) :e54–58. [CrossRef]
31. Styer L, Sullivan T, Parker M. 2011. Evaluation of an alternative supplemental testing strategy for HIV diagnosis by retrospective analysis of clinical HIV testing data. J Clin Virol 52S:S35–S40. [CrossRef]
32. Bennett B, Neumann D, Fordan S, Villaraza R, Crowe S, Gillis L. 2013. Performance of the new HIV-1/2 diagnostic algorithm in Florida’spublic health testing population: a review of the first five months ofutilization. J Clin Virol 58S:e29–e33. [CrossRef]
33. Manlutac A, Giesick J, McVay P. 2013. Identification of early HIV infections using the fourth generationAbbott ARCHITECT HIV Ag/Ab Combo chemiluminescentmicroparticle immunoassay (CIA) in San Diego County. J Clin Virol 58S:e44–e47. [CrossRef]
34. Bio-Rad-Laboratories. 2013. Package Insert—Multispot HIV-1/HIV-2 Rapid Test.
35. Karasi JC, Dziezuk F, Quennery L, Forster S, Reischl U, Colucci G, Schoener D, Seguin-Devaux C, Schmit JC. 2011. High correlation between the Roche COBAS(R) AmpliPrep/COBAS(R) TaqMan(R) HIV-1, v2.0 and the Abbott m2000 RealTime HIV-1 assays for quantification of viral load in HIV-1 B and non-B subtypes. J Clin Virol 52:181–186. [CrossRef]
36. Swenson LC, Cobb B, Geretti AM, Harrigan PR, Poljak M, Seguin-Devaux C, Verhofstede C, Wirden M, Amendola A, Boni J, Bourlet T, Huder JB, Karasi JC, Zidovec Lepej S, Lunar MM, Mukabayire O, Schuurman R, Tomazic J, Van Laethem K, Vandekerckhove L, Wensing AM, Tang Y-W, International Viral Load Assay Collaboration. 2014. Comparative performances of HIV-1 RNA load assays at low viral load levels: results of an international collaboration. J Clin Microbiol 52:517–523. [CrossRef]
37. Lofgren SM, Morrissey AB, Chevallier CC, Malabeja AI, Edmonds S, Amos B, Sifuna DJ, von Seidlein L, Schimana W, Stevens WS, Bartlett JA, Crump JA. 2009. Evaluation of a dried blood spot HIV-1 RNA program for early infant diagnosis and viral load monitoring at rural and remote healthcare facilities. AIDS 23:2459–2466. [CrossRef]
38. Fiscus SA, Brambilla D, Grosso L, Schock J, Cronin M. 1998. Quantitation of human immunodeficiency virus type 1 RNA in plasma by using blood dried on filter paper. J Clin Microbiol 36:258–260.
39. Lew J, Reichelderfer P, Fowler M, Bremer J, Carrol R, Cassol S, Chernoff D, Coombs R, Cronin M, Dickover R, Fiscus S, Herman S, Jackson B, Kornegay J, Kovacs A, McIntosh K, Meyer W, Michael N, Mofenson L, Moye J, Quinn T, Robb M, Vahey M, Weiser B, Yeghiazarian T for the TUBE Meeting Workshop Attendees. 1998. Determinations of levels of human immunodeficiency virus type 1 RNA in plasma: reassessment of parameters affecting assay outcome. J Clin Microbiol 36:1471–1479.
40. Havlir DV, Bassett R, Levitan D, Gilbert P, Tebas P, Collier AC, Hirsch MS, Ignacio C, Condra J, Günthard HF, Richman DD, Wong JK. 2001. Prevalence and predictive value of intermittent viremia with combination HIV therapy. JAMA 286:171–179. [CrossRef]
41. Lalama CM, Jennings C, Johnson VA, Coombs RW, McKinnon JE, Bremer JW, Cobb BR, Cloherty GA, Mellors JW, Ribaudo HJ, Group ACT, AIDS Clinical Trials Group. 2015. Comparison of three different FDA-approved plasma HIV-1 RNA assay platforms confirms the virologic failure endpoint of 200 copies per milliliter despite improved assay sensitivity. J Clin Microbiol 53:2659–2666. [CrossRef]
42. Abram ME, Ferris AL, Das K, Quinoñes O, Shao W, Tuske S, Alvord WG, Arnold E, Hughes SH. 2014. Mutations in HIV-1 reverse transcriptase affect the errors made in a single cycle of viral replication. J Virol 88:7589–7601. [CrossRef]
43. Kirchhoff F. 2010. Immune evasion and counteraction of restriction factors by HIV-1 and other primate lentiviruses. Cell Host Microbe 8:55–67. [CrossRef]
44. Wei X, Decker JM, Wang S, Hui H, Kappes JC, Wu X, Salazar-Gonzalez JF, Salazar MG, Kilby JM, Saag MS, Komarova NL, Nowak MA, Hahn BH, Kwong PD, Shaw GM. 2003. Antibody neutralization and escape by HIV-1. Nature 422:307–312. [CrossRef]
45. Larder BA, Kemp SD. 1989. Multiple mutations in HIV-1 reverse transcriptase confer high-level resistance to zidovudine (AZT). Science 246:1155–1158. [CrossRef]
46. Liu TF, Shafer RW. 2006. Web resources for HIV type 1 genotypic-resistance test interpretation. Clin Infect Dis 42:1608–1618. [CrossRef]
47. Simen BB, Braverman MS, Abbate I, Aerssens J, Bidet Y, Bouchez O, Gabriel C, Izopet J, Kessler HH, Stelzl E, Di Giallonardo F, Schlapbach R, Radonic A, Paredes R, Recordon-Pinson P, Sakwa J, St John EP, Schmitz-Agheguian GG, Metzner KJ, Däumer MP, Group HIVA, 454 HIV Alphastudy Group. 2014. An international multicenter study on HIV-1 drug resistance testing by 454 ultra-deep pyrosequencing. J Virol Methods 204:31–37. [CrossRef]
48. Pou C, Noguera-Julian M, Pérez-Álvarez S, García F, Delgado R, Dalmau D, Álvarez-Tejado M, Gonzalez D, Sayada C, Chueca N, Pulido F, Ibáñez L, Rodríguez C, Casadellà M, Santos JR, Ruiz L, Clotet B, Paredes R. 2014. Improved prediction of salvage antiretroviral therapy outcomes using ultrasensitive HIV-1 drug resistance testing. Clin Infect Dis 59:578–588. [CrossRef]
49. Lübke N, Di Cristanziano V, Sierra S, Knops E, Schülter E, Jensen B, Oette M, Lengauer T, Kaiser R. 2015. Proviral DNA as a Target for HIV-1 Resistance Analysis. Intervirology 58:184–189. [CrossRef]
50. Delaugerre C, Braun J, Charreau I, Delarue S, Nere ML, de Castro N, May T, Marchou B, Simon F, Molina JM, Aboulker JP, ANRS 138-EASIER study group. 2012. Comparison of resistance mutation patterns in historical plasma HIV RNA genotypes with those in current proviral HIV DNA genotypes among extensively treated patients with suppressed replication. HIV Med 13:517–525. [CrossRef]
51. Harrigan PR, Montaner JS, Wegner SA, Verbiest W, Miller V, Wood R, Larder BA. 2001. World-wide variation in HIV-1 phenotypic susceptibility in untreated individuals: biologically relevant values for resistance testing. AIDS 15:1671–1677. [CrossRef]
52. Petropoulos CJ, Parkin NT, Limoli KL, Lie YS, Wrin T, Huang W, Tian H, Smith D, Winslow GA, Capon DJ, Whitcomb JM. 2000. A novel phenotypic drug susceptibility assay for human immunodeficiency virus type 1. Antimicrob Agents Chemother 44:920–928. [CrossRef]
53. Aitken SC, Wallis CL, Stevens W, de Wit TR, Schuurman R. 2015. Stability of HIV-1 nucleic acids in dried blood spot samples for HIV-1 drug resistance genotyping. PLoS One 10:e0131541. [CrossRef]
54. McNulty A, Jennings C, Bennett D, Fitzgibbon J, Bremer JW, Ussery M, Kalish ML, Heneine W, García-Lerma JG. 2007. Evaluation of dried blood spots for human immunodeficiency virus type 1 drug resistance testing. J Clin Microbiol 45:517–521. [CrossRef]
55. Youngpairoj AS, Masciotra S, Garrido C, Zahonero N, de Mendoza C, García-Lerma JG. 2008. HIV-1 drug resistance genotyping from dried blood spots stored for 1 year at 4°C. J Antimicrob Chemother 61:1217–1220. [CrossRef]
56. Ziemniak C, Mengistu Y, Ruff A, Chen YH, Khaki L, Bedri A, Simen BB, Palumbo P, Eshleman SH, Persaud D. 2011. Use of dried-blood-spot samples and in-house assays to identify antiretroviral drug resistance in HIV-infected children in resource-constrained settings. J Clin Microbiol 49:4077–4082. [CrossRef]
57. Whitcomb JM, Huang W, Fransen S, Limoli K, Toma J, Wrin T, Chappey C, Kiss LD, Paxinos EE, Petropoulos CJ. 2007. Development and characterization of a novel single-cycle recombinant-virus assay to determine human immunodeficiency virus type 1 coreceptor tropism. Antimicrob Agents Chemother 51:566–575. [CrossRef]
58. Pou C, Codoñer FM, Thielen A, Bellido R, Pérez-Álvarez S, Cabrera C, Dalmau J, Curriu M, Lie Y, Noguera-Julian M, Puig J, Martínez-Picado J, Blanco J, Coakley E, Däumer M, Clotet B, Paredes R. 2013. HIV-1 tropism testing in subjects achieving undetectable HIV-1 RNA: diagnostic accuracy, viral evolution and compartmentalization. PLoS One 8:e67085. [CrossRef]
59. Branson BM, Owen SM. 2015. Human immunodeficiency virus. p. 1437. In Jorgensen JH, Pfaller MA, Carroll KC (ed), Manual of Clinical Microbiology, 11th ed. ASM Press, Washington, DC. [CrossRef]
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2016-07-01
2021-04-11

Abstract:

In this chapter we will discuss the diagnosis and monitoring of individuals with HIV infection. The application and interpretation of these tests does not change dramatically when used in the immunocompromised host. The principal approach to the diagnosis of HIV infection involves serologic testing, although nucleic acid amplification tests play an important role in the diagnosis of acute HIV infection. The algorithm for diagnosis of HIV continues to evolve with newer assays that are able to detect infection within an earlier timeframe after HIV transmission. Viral load testing for HIV-1 is the cornerstone for monitoring patients on antiretroviral therapy. Genotypic and phenotypic resistance tests are employed when antiretroviral resistance is suspected and results help guide therapy. The tropism assay must be performed to determine the efficacy of CCR5 chemokine receptor antagonists. Next-generation sequencing methods are an innovative approach to assessing archived antiretroviral resistance in patients with virologic suppression. The success of antiretroviral therapy with improved long-term outcomes has made transplantation in HIV-infected patients a reality.

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Human Immunodeficiency Virus
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Citation: Armstrong W, Guarner J, Kraft C, Caliendo A. 2016. Human immunodeficiency virus. microbiolspec 4(4): doi:10.1128/microbiolspec.DMIH2-0024-2015
/content/microbiolspec/10.1128/microbiolspec.DMIH2-0024-2015.fig1
FIGURE 1
Life cycle of HIV-1. Reprinted from reference 59 , with permission.
microbiolspec/4/4/DMIH2-0024-2015-fig1_thmb.gif
microbiolspec/4/4/DMIH2-0024-2015-fig1.gif
Image of FIGURE 1

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FIGURE 1

Life cycle of HIV-1. Reprinted from reference 59 , with permission.

Source: microbiolspec July 2016 vol. 4 no. 4 doi:10.1128/microbiolspec.DMIH2-0024-2015
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/content/microbiolspec/10.1128/microbiolspec.DMIH2-0024-2015.fig2
FIGURE 2
Timeline for detection of virus using different assays, as compared to Western blot.
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FIGURE 2

Timeline for detection of virus using different assays, as compared to Western blot.

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FIGURE 3
CDC and Association of Public Health Laboratories guideline for diagnosis of HIV infection.
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FIGURE 3

CDC and Association of Public Health Laboratories guideline for diagnosis of HIV infection.

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Tables

Human Immunodeficiency Virus
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Citation: Armstrong W, Guarner J, Kraft C, Caliendo A. 2016. Human immunodeficiency virus. microbiolspec 4(4): doi:10.1128/microbiolspec.DMIH2-0024-2015
/content/microbiolspec/10.1128/microbiolspec.DMIH2-0024-2015.T1
TABLE 1
Examples of HIV-1 serologic assays
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TABLE 1

Examples of HIV-1 serologic assays

Source: microbiolspec July 2016 vol. 4 no. 4 doi:10.1128/microbiolspec.DMIH2-0024-2015
/content/microbiolspec/10.1128/microbiolspec.DMIH2-0024-2015.T2
TABLE 2
Second-generation rapid HIV tests and second-generation rapid differentiation tests
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TABLE 2

Second-generation rapid HIV tests and second-generation rapid differentiation tests

Source: microbiolspec July 2016 vol. 4 no. 4 doi:10.1128/microbiolspec.DMIH2-0024-2015
/content/microbiolspec/10.1128/microbiolspec.DMIH2-0024-2015.T3
TABLE 3
FDA-approved real-time PCR tests for quantification of HIV-1 RNA
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TABLE 3

FDA-approved real-time PCR tests for quantification of HIV-1 RNA

Source: microbiolspec July 2016 vol. 4 no. 4 doi:10.1128/microbiolspec.DMIH2-0024-2015
/content/microbiolspec/10.1128/microbiolspec.DMIH2-0024-2015.T4
TABLE 4
Selected assays for resistance testing
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TABLE 4

Selected assays for resistance testing

Source: microbiolspec July 2016 vol. 4 no. 4 doi:10.1128/microbiolspec.DMIH2-0024-2015

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